Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2103337..2103866 Replicon chromosome
Accession NZ_CP092556
Organism Staphylococcus aureus strain VMRSA-WC062

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MKI64_RS10445 Protein ID WP_000621175.1
Coordinates 2103337..2103699 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MKI64_RS10450 Protein ID WP_000948331.1
Coordinates 2103696..2103866 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI64_RS10425 (MKI64_002067) 2100315..2101085 - 771 WP_001041111.1 RNA polymerase sigma factor SigB -
MKI64_RS10430 (MKI64_002068) 2101060..2101539 - 480 WP_001190829.1 anti-sigma B factor RsbW -
MKI64_RS10435 (MKI64_002069) 2101541..2101867 - 327 WP_001052491.1 anti-sigma factor antagonist -
MKI64_RS10440 (MKI64_002070) 2101986..2102987 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MKI64_RS10445 (MKI64_002071) 2103337..2103699 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKI64_RS10450 (MKI64_002072) 2103696..2103866 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MKI64_RS10455 (MKI64_002073) 2103951..2105099 - 1149 WP_001281154.1 alanine racemase -
MKI64_RS10460 (MKI64_002074) 2105165..2105524 - 360 WP_000581197.1 holo-ACP synthase -
MKI64_RS10465 (MKI64_002075) 2105528..2106019 - 492 WP_001205912.1 PH domain-containing protein -
MKI64_RS10470 (MKI64_002076) 2106006..2107589 - 1584 WP_001294626.1 PH domain-containing protein -
MKI64_RS10475 (MKI64_002077) 2107582..2108061 - 480 WP_001287087.1 hypothetical protein -
MKI64_RS10480 (MKI64_002078) 2108269..2108829 - 561 WP_001092411.1 K(+)-transporting ATPase subunit C -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-110)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T236573 WP_000621175.1 NZ_CP092556:c2103699-2103337 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T236573 NZ_LR698955:219888-220145 [Fusobacterium nucleatum]
ATGTTATTGACTTGGACTGATTTTGCATGGGAACAATATGAAGAATTACAGGAAAAAGATAAAAGACTTGTAAAAAAGAT
AAATATCCTTATTAAGGATATAAAAAGGAATGGTAATGAAAGAATAGGAAAACCAGAACCTTTACAACATGAGCTAAGTG
GCTATTGGAGTAGAAGAATTGATTATAAAAATAGATTAGTTTATAAGATATCAGATACTCAAATAATAATAGTTGCTTGT
GCAAATCATTATAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT236573 WP_000948331.1 NZ_CP092556:c2103866-2103696 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT236573 NZ_LR698955:219631-219888 [Fusobacterium nucleatum]
ATGATAGCAACTAATTATTCAGAGGTTAGAAATAATTTAAAAGCTTATTGTGATAAGGCAACAAAAGACTACGAAACTAT
AATTATTACAAGAAAAAATAATGAAAATGTTGTTTTGATGAGTGAAGAAGAATATAATAATCTTATGGAAAACCTTTATA
TAAGATCAAATCTTAAATATTATCAAAAACTTGTAGAAAGTATAAAAGAAGTTGAAAAGGGTAATGTTAAAGAACATGAT
TTAGTAGAGGTGGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References