Detailed information of TA system TA236571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1890421..1891197
• Replicon: chromosome
• Accession: NZ_CP092556
• Organism: Staphylococcus aureus strain VMRSA-WC062

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MKI64_RS09085
• Protein ID: WP_000031108.1
• Coordinates: 1890421..1890573 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MKI64_RS09090
• Protein ID: WP_001251224.1
• Coordinates: 1890598..1891197 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI64_RS09070 (MKI64_001799)	1886623..1887444	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MKI64_RS09075 (MKI64_001800)	1887897..1889282	-	1386	WP_000116228.1	class II fumarate hydratase	-
MKI64_RS09080 (MKI64_001801)	1889478..1889873	-	396	WP_000901023.1	hypothetical protein	-
MKI64_RS09085 (MKI64_001802)	1890421..1890573	-	153	WP_000031108.1	SAS053 family protein	Toxin
MKI64_RS09090 (MKI64_001803)	1890598..1891197	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MKI64_RS09095 (MKI64_001804)	1891356..1891826	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MKI64_RS09100 (MKI64_001805)	1891831..1892958	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
MKI64_RS09105 (MKI64_001806)	1893109..1893831	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MKI64_RS09110 (MKI64_001807)	1893824..1895281	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T236571 WP_000031108.1 NZ_CP092556:c1890573-1890421 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T236571 NZ_LR698953:1832225-1832548 [Bifidobacterium longum]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT236571 WP_001251224.1 NZ_CP092556:c1891197-1890598 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT236571 NZ_LR698953:1831993-1832238 [Bifidobacterium longum]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4