Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2102409..2102938 Replicon chromosome
Accession NZ_CP092554
Organism Staphylococcus aureus strain VMRSA-WC071

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MKI61_RS10440 Protein ID WP_000621175.1
Coordinates 2102409..2102771 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MKI61_RS10445 Protein ID WP_000948331.1
Coordinates 2102768..2102938 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI61_RS10420 (MKI61_002067) 2099387..2100157 - 771 WP_001041111.1 RNA polymerase sigma factor SigB -
MKI61_RS10425 (MKI61_002068) 2100132..2100611 - 480 WP_001190829.1 anti-sigma B factor RsbW -
MKI61_RS10430 (MKI61_002069) 2100613..2100939 - 327 WP_001052491.1 anti-sigma factor antagonist -
MKI61_RS10435 (MKI61_002070) 2101058..2102059 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MKI61_RS10440 (MKI61_002071) 2102409..2102771 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKI61_RS10445 (MKI61_002072) 2102768..2102938 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MKI61_RS10450 (MKI61_002073) 2103023..2104171 - 1149 WP_001281154.1 alanine racemase -
MKI61_RS10455 (MKI61_002074) 2104237..2104596 - 360 WP_000581197.1 holo-ACP synthase -
MKI61_RS10460 (MKI61_002075) 2104600..2105091 - 492 WP_001205912.1 PH domain-containing protein -
MKI61_RS10465 (MKI61_002076) 2105078..2106661 - 1584 WP_001294626.1 PH domain-containing protein -
MKI61_RS10470 (MKI61_002077) 2106654..2107133 - 480 WP_001287087.1 hypothetical protein -
MKI61_RS10475 (MKI61_002078) 2107341..2107901 - 561 WP_001092411.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T236561 WP_000621175.1 NZ_CP092554:c2102771-2102409 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T236561 NZ_LR698844:2720566-2720715 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTTAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT236561 WP_000948331.1 NZ_CP092554:c2102938-2102768 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT236561 NZ_LR698844:c2720832-2720648 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References