Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2120699..2121213 Replicon chromosome
Accession NZ_CP092552
Organism Staphylococcus aureus strain VMRSA-WC081

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI53_RS10500 Protein ID WP_000581792.1
Coordinates 2120699..2120908 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI53_RS10505 Protein ID WP_000138932.1
Coordinates 2120920..2121213 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI53_RS10480 (MKI53_002080) 2116207..2117565 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI53_RS10485 (MKI53_002081) 2117580..2118650 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI53_RS10490 (MKI53_002082) 2118968..2120170 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI53_RS10495 (MKI53_002083) 2120434..2120571 - 138 WP_000828354.1 Lmo0850 family protein -
MKI53_RS10500 (MKI53_002084) 2120699..2120908 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI53_RS10505 (MKI53_002085) 2120920..2121213 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI53_RS10510 (MKI53_002086) 2121379..2122872 + 1494 WP_000571554.1 cardiolipin synthase -
MKI53_RS10515 (MKI53_002087) 2122900..2123547 + 648 WP_001187633.1 HD domain-containing protein -
MKI53_RS10520 (MKI53_002088) 2124460..2125332 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI53_RS10525 (MKI53_002089) 2125418..2126059 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236550 WP_000581792.1 NZ_CP092552:c2120908-2120699 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236550 NZ_LR657304:1573359-1574228 [Pseudomonas aeruginosa PAK]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236550 WP_000138932.1 NZ_CP092552:c2121213-2120920 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236550 NZ_LR657304:1574221-1575054 [Pseudomonas aeruginosa PAK]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References