Detailed information of TA system TA236549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2099440..2099969
• Replicon: chromosome
• Accession: NZ_CP092552
• Organism: Staphylococcus aureus strain VMRSA-WC081

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI53_RS10400
• Protein ID: WP_000621175.1
• Coordinates: 2099440..2099802 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI53_RS10405
• Protein ID: WP_000948331.1
• Coordinates: 2099799..2099969 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI53_RS10380 (MKI53_002061)	2096418..2097188	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI53_RS10385 (MKI53_002062)	2097163..2097642	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI53_RS10390 (MKI53_002063)	2097644..2097970	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI53_RS10395 (MKI53_002064)	2098089..2099090	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI53_RS10400 (MKI53_002065)	2099440..2099802	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI53_RS10405 (MKI53_002066)	2099799..2099969	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI53_RS10410 (MKI53_002067)	2100054..2101202	-	1149	WP_001281154.1	alanine racemase	-
MKI53_RS10415 (MKI53_002068)	2101268..2101627	-	360	WP_000581197.1	holo-ACP synthase	-
MKI53_RS10420 (MKI53_002069)	2101631..2102122	-	492	WP_001205912.1	PH domain-containing protein	-
MKI53_RS10425 (MKI53_002070)	2102109..2103692	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI53_RS10430 (MKI53_002071)	2103685..2104164	-	480	WP_001287087.1	hypothetical protein	-
MKI53_RS10435 (MKI53_002072)	2104372..2104932	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236549 WP_000621175.1 NZ_CP092552:c2099802-2099440 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236549 NZ_LR657304:c142034-141753 [Pseudomonas aeruginosa PAK]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGAAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236549 WP_000948331.1 NZ_CP092552:c2099969-2099799 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236549 NZ_LR657304:c142258-142031 [Pseudomonas aeruginosa PAK]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAAAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1