Detailed information of TA system TA236548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_XRE(antitoxin)
• Location: 1959576..1960373
• Replicon: chromosome
• Accession: NZ_CP092552
• Organism: Staphylococcus aureus strain VMRSA-WC081

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MKI53_RS09645
• Protein ID: WP_000525008.1
• Coordinates: 1959912..1960373 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C2LD36
• Locus tag: MKI53_RS09640
• Protein ID: WP_001260487.1
• Coordinates: 1959576..1959899 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI53_RS09575 (MKI53_001902)	1954893..1955426	-	534	WP_001004326.1	host-nuclease inhibitor Gam family protein	-
MKI53_RS09580 (MKI53_001903)	1955419..1955670	-	252	WP_000683290.1	hypothetical protein	-
MKI53_RS09585 (MKI53_001904)	1955680..1955940	-	261	WP_000291481.1	DUF1108 family protein	-
MKI53_RS09590 (MKI53_001905)	1955945..1956247	-	303	WP_000163430.1	DUF2482 family protein	-
MKI53_RS09595 (MKI53_001906)	1956340..1956501	-	162	WP_000048124.1	DUF1270 family protein	-
MKI53_RS09600 (MKI53_001907)	1956494..1956709	-	216	WP_000977384.1	hypothetical protein	-
MKI53_RS09605 (MKI53_001908)	1956723..1957172	-	450	WP_000993183.1	hypothetical protein	-
MKI53_RS09610 (MKI53_001909)	1957212..1957436	-	225	WP_000187184.1	hypothetical protein	-
MKI53_RS09615 (MKI53_001910)	1957437..1958228	-	792	WP_001157019.1	phage antirepressor KilAC domain-containing protein	-
MKI53_RS09620 (MKI53_001911)	1958285..1958524	+	240	WP_000438628.1	hypothetical protein	-
MKI53_RS09625 (MKI53_001912)	1958514..1958693	-	180	WP_000393082.1	hypothetical protein	-
MKI53_RS09630 (MKI53_001913)	1958708..1959151	-	444	WP_000435356.1	hypothetical protein	-
MKI53_RS09635 (MKI53_001914)	1959164..1959412	-	249	WP_000272859.1	helix-turn-helix transcriptional regulator	-
MKI53_RS09640 (MKI53_001915)	1959576..1959899	+	324	WP_001260487.1	helix-turn-helix transcriptional regulator	Antitoxin
MKI53_RS09645 (MKI53_001916)	1959912..1960373	+	462	WP_000525008.1	hypothetical protein	Toxin
MKI53_RS09650 (MKI53_001917)	1960557..1961078	+	522	WP_001102444.1	hypothetical protein	-
MKI53_RS09655 (MKI53_001918)	1961071..1961541	+	471	WP_000183249.1	hypothetical protein	-
MKI53_RS09660 (MKI53_001919)	1961611..1962657	+	1047	WP_001145732.1	tyrosine-type recombinase/integrase	-
MKI53_RS09665 (MKI53_001920)	1962702..1963847	+	1146	WP_001245449.1	radical SAM/CxCxxxxC motif protein YfkAB	-
MKI53_RS09670 (MKI53_001921)	1964107..1964637	-	531	WP_000184383.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1918204..1964637	46433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18034.35 Da
• Isoelectric Point: 4.6899

>T236548 WP_000525008.1 NZ_CP092552:1959912-1960373 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSNFNNRKFENYAR
RHGFISAVPLREIVEAHNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T236548 NZ_LR655210:c1985025-1984534 [Bifidobacterium longum subsp. infantis]
ATGACCGACGTTTCCATGCAGCGTATTTTGCTTGATACCAATGTACTGTTGGACTATTTGCTCCATCGCGATGATCACGC
CAAGATGGCGGAAGCGGTTATGGAACTTGGCGCGAAGAACAACGTTACGTTGTTGTGCGCGTCATTGTCGCTGAAGGATA
TCGCATACCTATTATCATCGGCGATTCGTAGGGAATTCAAACCGAATGAATCGGAAGTGGAGAATTTCACTCGCAACTTT
CTGTCGTCACGGGTGCCATGGCGTTGTATCGAGCAGGTGAAGGAGATGTGTGACATTGTCGCTGTCGATGAGTCGACATG
TGATAAGGCTTTTTCCCTGCAAAAACGGCATCGGGATTTTGAAGATGATCTGATCATCGTTGCCGCGCAACAATCCGGTG
CCAACTGTGTGGTCACATCGGATGCCGAATTAATCAGTCATTTTCCCGAATATTGCAAAACACCGGCTGAAATCGTGGCG
GCGCTGGAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12431.09 Da
• Isoelectric Point: 9.3811

>AT236548 WP_001260487.1 NZ_CP092552:1959576-1959899 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 324 bp

>AT236548 NZ_LR655210:c1985387-1985025 [Bifidobacterium longum subsp. infantis]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTATACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGAGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGCACCGGATACGTGCACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGACAAAATGTACGA
CGAGCAACTTGATACGTATTACAACATGAAGAGGGCCGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C2LD36