Detailed information of TA system TA236547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1886535..1887311
• Replicon: chromosome
• Accession: NZ_CP092552
• Organism: Staphylococcus aureus strain VMRSA-WC081

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MKI53_RS09040
• Protein ID: WP_000031108.1
• Coordinates: 1886535..1886687 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MKI53_RS09045
• Protein ID: WP_001251224.1
• Coordinates: 1886712..1887311 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI53_RS09025 (MKI53_001793)	1882737..1883558	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MKI53_RS09030 (MKI53_001794)	1884011..1885396	-	1386	WP_000116228.1	class II fumarate hydratase	-
MKI53_RS09035 (MKI53_001795)	1885592..1885987	-	396	WP_000901023.1	hypothetical protein	-
MKI53_RS09040 (MKI53_001796)	1886535..1886687	-	153	WP_000031108.1	SAS053 family protein	Toxin
MKI53_RS09045 (MKI53_001797)	1886712..1887311	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MKI53_RS09050 (MKI53_001798)	1887470..1887940	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MKI53_RS09055 (MKI53_001799)	1887945..1889072	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
MKI53_RS09060 (MKI53_001800)	1889223..1889945	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MKI53_RS09065 (MKI53_001801)	1889938..1891395	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T236547 WP_000031108.1 NZ_CP092552:c1886687-1886535 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T236547 NZ_LR655210:c1524658-1524365 [Bifidobacterium longum subsp. infantis]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTATTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT236547 WP_001251224.1 NZ_CP092552:c1887311-1886712 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT236547 NZ_LR655210:c1524931-1524662 [Bifidobacterium longum subsp. infantis]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATTGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCCCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGTAATCTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4