Detailed information of TA system TA236544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 849031..849508
• Replicon: chromosome
• Accession: NZ_CP092552
• Organism: Staphylococcus aureus strain VMRSA-WC081

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MKI53_RS04060
• Protein ID: WP_000052076.1
• Coordinates: 849031..849219 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MKI53_RS04065
• Protein ID: WP_001059690.1
• Coordinates: 849248..849508 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI53_RS04030 (MKI53_000797)	845025..845546	+	522	WP_000824482.1	hypothetical protein	-
MKI53_RS04035 (MKI53_000798)	845887..846573	+	687	WP_000141557.1	thermonuclease family protein	-
MKI53_RS04040 (MKI53_000799)	846930..847130	+	201	WP_001059082.1	cold-shock protein	-
MKI53_RS04045 (MKI53_000800)	847622..847840	-	219	WP_000005886.1	hypothetical protein	-
MKI53_RS04050 (MKI53_000801)	847902..848186	-	285	WP_001004399.1	hypothetical protein	-
MKI53_RS04055 (MKI53_000802)	848274..848843	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
MKI53_RS04060 (MKI53_000803)	849031..849219	-	189	WP_000052076.1	hypothetical protein	Toxin
MKI53_RS04065 (MKI53_000804)	849248..849508	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MKI53_RS04070 (MKI53_000805)	849849..850052	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MKI53_RS04075 (MKI53_000806)	850049..850285	-	237	WP_000349581.1	hypothetical protein	-
MKI53_RS04080 (MKI53_000807)	850510..851091	+	582	WP_001140693.1	histidine phosphatase family protein	-
MKI53_RS04085 (MKI53_000808)	851164..851781	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
MKI53_RS04090 (MKI53_000809)	851936..852430	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MKI53_RS04095 (MKI53_000810)	852829..853251	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
MKI53_RS04100 (MKI53_000811)	853399..854115	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T236544 WP_000052076.1 NZ_CP092552:c849219-849031 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T236544 NZ_LR655210:c1257235-1256912 [Bifidobacterium longum subsp. infantis]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT236544 WP_001059690.1 NZ_CP092552:c849508-849248 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT236544 NZ_LR655210:c1257467-1257222 [Bifidobacterium longum subsp. infantis]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56