Detailed information of TA system TA236543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 2697860..2698379
• Replicon: chromosome
• Accession: NZ_CP092550
• Organism: Staphylococcus aureus strain VMRSA-WC082

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: MKI63_RS13530
• Protein ID: WP_000588503.1
• Coordinates: 2697860..2698117 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: MKI63_RS13535
• Protein ID: WP_000388479.1
• Coordinates: 2698110..2698379 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI63_RS13500 (MKI63_002675)	2692900..2693190	-	291	WP_000026576.1	hypothetical protein	-
MKI63_RS13505 (MKI63_002676)	2693326..2693505	-	180	WP_001021554.1	hypothetical protein	-
MKI63_RS13510 (MKI63_002677)	2693502..2693852	-	351	WP_000568378.1	hypothetical protein	-
MKI63_RS13515 (MKI63_002678)	2693845..2695170	-	1326	WP_000969590.1	Y-family DNA polymerase	-
MKI63_RS13520 (MKI63_002679)	2695620..2696234	+	615	WP_001261742.1	recombinase family protein	-
MKI63_RS13525 (MKI63_002680)	2696399..2697223	-	825	WP_001809248.1	hypothetical protein	-
MKI63_RS13530 (MKI63_002681)	2697860..2698117	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
MKI63_RS13535 (MKI63_002682)	2698110..2698379	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI63_RS13540 (MKI63_002683)	2698510..2698689	-	180	WP_001120991.1	hypothetical protein	-
MKI63_RS13545 (MKI63_002684)	2699070..2699366	-	297	WP_200857964.1	hypothetical protein	-
MKI63_RS13550 (MKI63_002685)	2699712..2700392	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
MKI63_RS13555 (MKI63_002686)	2700542..2701714	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
MKI63_RS13560 (MKI63_002687)	2701844..2703283	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aph(3')-III / msr(A) / mph(C) / tet(S) / ant(6)-Ia / erm(B) / aac(6')-aph(2'') / VanHAX	-	2591945..2753350	161405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T236543 WP_000588503.1 NZ_CP092550:c2698117-2697860 [Staphylococcus aureus]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T236543 NZ_LR655210:241220-241510 [Bifidobacterium longum subsp. infantis]
ATGAGCGGCATCCGGTACACGGTCAAGACCACGTCCAGATTCAGGAAGGACTTCAAACTCGCCAGACGACGCGGACTGGA
CGCCGGCCTTTTCAAACAGGTTGTATCCATTCTGTCCGAGGGCGGCACACTGCCCGACCGGTATCATGACCATGCGCTTA
CAGGCAATATGACCGGCTTCAGGGAGTGCTATATCACACCAGACCTGCTGTTGGTCTATCTCATAGAGAAGGACGTGCTG
GTGCTCACTCTGACCCGAACGGGCACCCATAGTGGCCTTTTCGGAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT236543 WP_000388479.1 NZ_CP092550:c2698379-2698110 [Staphylococcus aureus]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT236543 NZ_LR655210:240945-241223 [Bifidobacterium longum subsp. infantis]
ATGAGCGGAACGACAACCACCGTCAACGTCCGAATGAACGCTGAGCTGAAAAAACAAGCCGAGGAACTGTTCGGGGAGCT
GGGCATGAACCTGACCACCGCAGTCAACATATTCATCCGCCAATCCCTGCGACAGGGCGGCATACCGTTCAACGTCTCGT
TGGATGAGCCGAACAGGGAGACCGTCTCCGCCATGTTGGAGGCGAAGCGAATCGCAAGGGACGGGAACGTGAAGGGCTAC
CGGGACCTGAACGCCTTGTTCAAGGACTTGGATGCATGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7