Detailed information of TA system TA236537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102699..2103228
• Replicon: chromosome
• Accession: NZ_CP092550
• Organism: Staphylococcus aureus strain VMRSA-WC082

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI63_RS10450
• Protein ID: WP_000621175.1
• Coordinates: 2102699..2103061 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI63_RS10455
• Protein ID: WP_000948331.1
• Coordinates: 2103058..2103228 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI63_RS10430 (MKI63_002066)	2099677..2100447	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI63_RS10435 (MKI63_002067)	2100422..2100901	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI63_RS10440 (MKI63_002068)	2100903..2101229	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI63_RS10445 (MKI63_002069)	2101348..2102349	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI63_RS10450 (MKI63_002070)	2102699..2103061	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI63_RS10455 (MKI63_002071)	2103058..2103228	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI63_RS10460 (MKI63_002072)	2103313..2104461	-	1149	WP_001281154.1	alanine racemase	-
MKI63_RS10465 (MKI63_002073)	2104527..2104886	-	360	WP_000581197.1	holo-ACP synthase	-
MKI63_RS10470 (MKI63_002074)	2104890..2105381	-	492	WP_001205912.1	PH domain-containing protein	-
MKI63_RS10475 (MKI63_002075)	2105368..2106951	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI63_RS10480 (MKI63_002076)	2106944..2107423	-	480	WP_001287087.1	hypothetical protein	-
MKI63_RS10485 (MKI63_002077)	2107631..2108191	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236537 WP_000621175.1 NZ_CP092550:c2103061-2102699 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236537 NZ_LR595897:1183245-1184150 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCATCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCGAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGAATCGCAGGCGAGCGGCTGGCGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGATGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236537 WP_000948331.1 NZ_CP092550:c2103228-2103058 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236537 NZ_LR595897:1184147-1185001 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCGGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCGTTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1