Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2499240..2499763 Replicon chromosome
Accession NZ_CP092547
Organism Staphylococcus aureus strain VMRSA-WC083

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag MKI66_RS12510 Protein ID WP_000074049.1
Coordinates 2499240..2499506 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag MKI66_RS12515 Protein ID WP_000587616.1
Coordinates 2499506..2499763 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI66_RS12490 (MKI66_002471) 2494547..2495734 - 1188 WP_000026189.1 MFS transporter -
MKI66_RS12495 (MKI66_002472) 2496130..2496906 - 777 WP_000072149.1 iron export ABC transporter permease subunit FetB -
MKI66_RS12500 (MKI66_002473) 2496899..2497561 - 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
MKI66_RS12505 (MKI66_002474) 2497809..2498885 - 1077 WP_001076673.1 M42 family metallopeptidase -
MKI66_RS12510 (MKI66_002475) 2499240..2499506 - 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
MKI66_RS12515 (MKI66_002476) 2499506..2499763 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MKI66_RS12520 (MKI66_002477) 2500122..2500577 + 456 WP_000732076.1 DUF1307 domain-containing protein -
MKI66_RS12525 (MKI66_002478) 2500745..2502310 - 1566 WP_000143492.1 FMN-binding glutamate synthase family protein -
MKI66_RS12530 (MKI66_002479) 2502505..2503254 + 750 WP_000072461.1 CPBP family lipoprotein N-acylation protein LnsB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T236530 WP_000074049.1 NZ_CP092547:c2499506-2499240 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T236530 NZ_LR595857:c278590-278255 [Streptococcus sp. NCTC 11567]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATACGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGGGTTGTTATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT236530 WP_000587616.1 NZ_CP092547:c2499763-2499506 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT236530 NZ_LR595857:c278867-278580 [Streptococcus sp. NCTC 11567]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References