Detailed information of TA system TA023653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961917..962476
• Replicon: chromosome
• Accession: NC_012441
• Organism: Brucella melitensis ATCC 23457

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BMEA_RS04675
• Protein ID: WP_002966807.1
• Coordinates: 962231..962476 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: BMEA_RS04670
• Protein ID: WP_004683740.1
• Coordinates: 961917..962231 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMEA_RS04625	957621..957824	-	204	WP_004683737.1	hypothetical protein	-
BMEA_RS04630	957827..958042	-	216	WP_005968947.1	hypothetical protein	-
BMEA_RS04635	958039..958242	-	204	WP_002964088.1	hypothetical protein	-
BMEA_RS04640	958292..958997	+	706	Protein_915	hypothetical protein	-
BMEA_RS04645	958994..959224	+	231	WP_002964091.1	hypothetical protein	-
BMEA_RS04650	959221..959496	+	276	WP_002964092.1	hypothetical protein	-
BMEA_RS04655	959519..959995	+	477	WP_002966806.1	hypothetical protein	-
BMEA_RS04660	960341..961051	+	711	WP_004683739.1	porin family protein	-
BMEA_RS04665	961399..961569	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BMEA_RS04670	961917..962231	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
BMEA_RS04675	962231..962476	-	246	WP_002966807.1	BrnT family toxin	Toxin
BMEA_RS04680	962609..963247	+	639	Protein_923	tRNA-guanine transglycosylase	-
BMEA_RS17645	963317..964075	+	759	Protein_924	IS5 family transposase	-
BMEA_RS04690	964076..964768	+	693	WP_006135935.1	hypothetical protein	-
BMEA_RS04695	965180..966631	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955849..969947	14098
	-	flank	IS/Tn	-	-	963317..963685	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T23653 WP_002966807.1 NC_012441:c962476-962231 [Brucella melitensis ATCC 23457]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T23653 NC_012441:c962476-962231 [Brucella melitensis ATCC 23457]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT23653 WP_004683740.1 NC_012441:c962231-961917 [Brucella melitensis ATCC 23457]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT23653 NC_012441:c962231-961917 [Brucella melitensis ATCC 23457]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1