Detailed information of TA system TA236526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2123877..2124391
• Replicon: chromosome
• Accession: NZ_CP092547
• Organism: Staphylococcus aureus strain VMRSA-WC083

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: MKI66_RS10555
• Protein ID: WP_000581792.1
• Coordinates: 2123877..2124086 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: MKI66_RS10560
• Protein ID: WP_000138932.1
• Coordinates: 2124098..2124391 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI66_RS10535 (MKI66_002085)	2119385..2120743	-	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
MKI66_RS10540 (MKI66_002086)	2120758..2121828	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
MKI66_RS10545 (MKI66_002087)	2122146..2123348	+	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
MKI66_RS10550 (MKI66_002088)	2123612..2123749	-	138	WP_000828354.1	Lmo0850 family protein	-
MKI66_RS10555 (MKI66_002089)	2123877..2124086	-	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
MKI66_RS10560 (MKI66_002090)	2124098..2124391	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
MKI66_RS10565 (MKI66_002091)	2124557..2126050	+	1494	WP_000571554.1	cardiolipin synthase	-
MKI66_RS10570 (MKI66_002092)	2126078..2126725	+	648	WP_001187633.1	HD domain-containing protein	-
MKI66_RS10575 (MKI66_002093)	2127638..2128510	-	873	WP_000725802.1	membrane protein insertase YidC	-
MKI66_RS10580 (MKI66_002094)	2128596..2129237	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T236526 WP_000581792.1 NZ_CP092547:c2124086-2123877 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T236526 NZ_LR595855:2904457-2904918 [Raoultella terrigena]
GTGATCCTCTACCGCCTGACTAAAAGCCGCTTTCTGACGACCGCCTGGTCTGGTTTTGGGGCGAAAGAGGCCGGCGGCCG
CTGGAACAGCGTTGGCACGGCGATGGTCTACTTGTCGGAAGCCGCTTCGCTGACCATGCTGGAAACGCTGGTGCACATCA
ATGCTCCGCAGCTGCTGGATGATTTCACGCTGCTGAGCATTGACGTACCGGACGACCAGGTGCTGACCGTCGATCCGGCC
TCACTCCCCGCGGACTGGGCCCGCGAGGATGCGCCAGCCCGGCTGGCACGCTACGGTGACGAGTGGGCCGCGAGAGCGGC
ATCAGCGGCGTTACGCGTACCGAGCGCCCTGTCGCCGGTGGAGTATAACTATCTATTCAATCCTCAACACCCTGCCGTTT
TCGATCTGCTGGCAAGCGTAAAGAGGATCCCCTTCCGCTTTGATACTCGCCTTAAGGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT236526 WP_000138932.1 NZ_CP092547:c2124391-2124098 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT236526 NZ_LR595855:2904017-2904460 [Raoultella terrigena]
ATGAAAGTCTATTCCCCGACCGAGAACGCAGAAAAAAATCTTAGCCCCTGGCACGCCATCGGCCTCGACGCTGAGGACGG
CATTGCGCTGATGGGTAAAATTAGCCACGGGCTGGAAGGTTCCGTCGCCCGGCAAATTTCCGCATGGGCGGAGATCACCC
CTGCCGAACTGCGCAAAATGTCAGGCATTGCGAATACCACCTTTAACCGCAGCATTAAATCGCGCTTTTCCGCCGACCAA
AGCGAGCGCCTGGTGCGCATTCTTCGCGTTATCGAGCGGGCGGTGGAGCTGTTCGAAGGTGATAAAGAGGCGGCGCAGAA
GTGGATGAATGAACCCAATCGCGCGCTCGGCTGGAAAACGCCGGCCGAGGTGGTCTCTTCTGAAACCGGCGCGCTGGAGG
TGATGCGCCTGATCACGCGCGTGGAGCACGGGGTCTATTCGTGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13