Detailed information of TA system TA236525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102618..2103147
• Replicon: chromosome
• Accession: NZ_CP092547
• Organism: Staphylococcus aureus strain VMRSA-WC083

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI66_RS10455
• Protein ID: WP_000621175.1
• Coordinates: 2102618..2102980 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI66_RS10460
• Protein ID: WP_000948331.1
• Coordinates: 2102977..2103147 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI66_RS10435 (MKI66_002066)	2099596..2100366	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI66_RS10440 (MKI66_002067)	2100341..2100820	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI66_RS10445 (MKI66_002068)	2100822..2101148	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI66_RS10450 (MKI66_002069)	2101267..2102268	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI66_RS10455 (MKI66_002070)	2102618..2102980	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI66_RS10460 (MKI66_002071)	2102977..2103147	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI66_RS10465 (MKI66_002072)	2103232..2104380	-	1149	WP_001281154.1	alanine racemase	-
MKI66_RS10470 (MKI66_002073)	2104446..2104805	-	360	WP_000581197.1	holo-ACP synthase	-
MKI66_RS10475 (MKI66_002074)	2104809..2105300	-	492	WP_001205912.1	PH domain-containing protein	-
MKI66_RS10480 (MKI66_002075)	2105287..2106870	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI66_RS10485 (MKI66_002076)	2106863..2107342	-	480	WP_001287087.1	hypothetical protein	-
MKI66_RS10490 (MKI66_002077)	2107550..2108110	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236525 WP_000621175.1 NZ_CP092547:c2102980-2102618 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236525 NZ_LR595855:2827949-2828434 [Raoultella terrigena]
GTGGGACATGTAACAGCACCAGAACCTCTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGACTGTGCTCGA
TGACTGGTTAAAGCACAAAGGCCTTAAAAACCAGGCTCTCGGAGCCGCCAGAACGTTTGTGGTATGTAAGAAAGATACAA
AGCAAATAGCTGGCTTTTACTCTCTGGCCACCGGGAGCGTCAATCATACTGAAGCGACAGGGAATCTTCGGCGTAATATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGCCTTGCTGTCGATCTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGAACATTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236525 WP_000948331.1 NZ_CP092547:c2103147-2102977 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236525 NZ_LR595855:2827695-2827961 [Raoultella terrigena]
ATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATAGATGCGGCTGCGGAAATCCTCCA
CAAGTCACGTACAGATTTCATTCTTGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCCTCGATCGCCGGGTATTTAACT
TTAACGATGAGCAATATGCGGAGTTCATCGATATGCTTGATGCACCTGTCGCAGATGATCCCATCATCGAAAAACTGCTG
GCAAGAAAACCCCAGTGGGACATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1