Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1889734..1890510 Replicon chromosome
Accession NZ_CP092547
Organism Staphylococcus aureus strain VMRSA-WC083

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag MKI66_RS09100 Protein ID WP_000031108.1
Coordinates 1889734..1889886 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag MKI66_RS09105 Protein ID WP_001251224.1
Coordinates 1889911..1890510 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI66_RS09085 (MKI66_001798) 1885936..1886757 + 822 WP_000669376.1 RluA family pseudouridine synthase -
MKI66_RS09090 (MKI66_001799) 1887210..1888595 - 1386 WP_000116228.1 class II fumarate hydratase -
MKI66_RS09095 (MKI66_001800) 1888791..1889186 - 396 WP_000901023.1 hypothetical protein -
MKI66_RS09100 (MKI66_001801) 1889734..1889886 - 153 WP_000031108.1 SAS053 family protein Toxin
MKI66_RS09105 (MKI66_001802) 1889911..1890510 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
MKI66_RS09110 (MKI66_001803) 1890669..1891139 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
MKI66_RS09115 (MKI66_001804) 1891144..1892271 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
MKI66_RS09120 (MKI66_001805) 1892422..1893144 - 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
MKI66_RS09125 (MKI66_001806) 1893137..1894594 - 1458 WP_000649908.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T236523 WP_000031108.1 NZ_CP092547:c1889886-1889734 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T236523 NZ_LR595855:2041799-2041902 [Raoultella terrigena]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT236523 WP_001251224.1 NZ_CP092547:c1890510-1889911 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT236523 NZ_LR595855:c2041906-2041764 [Raoultella terrigena]
AGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGCGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References