Detailed information of TA system TA236520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 852259..852736
• Replicon: chromosome
• Accession: NZ_CP092547
• Organism: Staphylococcus aureus strain VMRSA-WC083

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MKI66_RS04115
• Protein ID: WP_000052076.1
• Coordinates: 852259..852447 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MKI66_RS04120
• Protein ID: WP_001059690.1
• Coordinates: 852476..852736 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI66_RS04085 (MKI66_000802)	848253..848774	+	522	WP_000824482.1	hypothetical protein	-
MKI66_RS04090 (MKI66_000803)	849115..849801	+	687	WP_000141557.1	thermonuclease family protein	-
MKI66_RS04095 (MKI66_000804)	850158..850358	+	201	WP_001059082.1	cold-shock protein	-
MKI66_RS04100 (MKI66_000805)	850850..851068	-	219	WP_000005886.1	hypothetical protein	-
MKI66_RS04105 (MKI66_000806)	851130..851414	-	285	WP_001004399.1	hypothetical protein	-
MKI66_RS04110 (MKI66_000807)	851502..852071	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
MKI66_RS04115 (MKI66_000808)	852259..852447	-	189	WP_000052076.1	hypothetical protein	Toxin
MKI66_RS04120 (MKI66_000809)	852476..852736	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MKI66_RS04125 (MKI66_000810)	853077..853280	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MKI66_RS04130 (MKI66_000811)	853277..853513	-	237	WP_000349581.1	hypothetical protein	-
MKI66_RS04135 (MKI66_000812)	853738..854319	+	582	WP_001140693.1	histidine phosphatase family protein	-
MKI66_RS04140 (MKI66_000813)	854392..855009	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
MKI66_RS04145 (MKI66_000814)	855164..855658	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MKI66_RS04150 (MKI66_000815)	856057..856479	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
MKI66_RS04155 (MKI66_000816)	856627..857343	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	818583..863335	44752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T236520 WP_000052076.1 NZ_CP092547:c852447-852259 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T236520 NZ_LR595848:c1642624-1642370 [Streptococcus pneumoniae]
ATGCTGCTCAAGTTTACAGAAGATGCCTGGGCAGATTATTGCTACTGGCAAAATCAGGATAAGAAAACGTTAAAAAGAAT
CAATAAACTAATCAAGGATATTCAACGTGATCCCTTTACAGGAATAGGTAAACCAGAACCACTCAAATATGATTACCAAG
GAGCCTGGTCACGGCGTATTGATGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT236520 WP_001059690.1 NZ_CP092547:c852736-852476 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT236520 NZ_LR595848:c1642882-1642628 [Streptococcus pneumoniae]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGATGAGGACATTGTAGTCCTTTCAAAGAGTGAGTGGGATAGTATCCAAGAAACCCTGA
GAATTGCTCAAAATAAGGAACTTTCTGATAAGGTTTTGCGAGGAATGGCTCAAGTTCGTGCTGGAAGTACTCAGGTCCAT
GTTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56