Detailed information of TA system TA236519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 71271..71790
• Replicon: plasmid unnamed1
• Accession: NZ_CP092545
• Organism: Staphylococcus aureus strain VMRSA-WC102

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: MKI60_RS14455
• Protein ID: WP_000588503.1
• Coordinates: 71533..71790 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: MKI60_RS14450
• Protein ID: WP_000388479.1
• Coordinates: 71271..71540 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI60_RS14415 (MKI60_002867)	66842..67258	-	417	WP_001232859.1	hypothetical protein	-
MKI60_RS14420 (MKI60_002868)	67261..67545	-	285	WP_000353844.1	hypothetical protein	-
MKI60_RS14425 (MKI60_002869)	67911..68135	+	225	WP_000410576.1	hypothetical protein	-
MKI60_RS14430 (MKI60_002871)	69258..69938	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
MKI60_RS14435	70014..70580	+	567	Protein_80	hypothetical protein	-
MKI60_RS14440	70745..70945	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
MKI60_RS14445 (MKI60_002873)	70961..71140	+	180	WP_001120991.1	hypothetical protein	-
MKI60_RS14450 (MKI60_002874)	71271..71540	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI60_RS14455 (MKI60_002875)	71533..71790	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
MKI60_RS14460 (MKI60_002876)	72427..73251	+	825	WP_001809248.1	hypothetical protein	-
MKI60_RS14465 (MKI60_002877)	73416..74030	-	615	WP_001261742.1	recombinase family protein	-
MKI60_RS14470 (MKI60_002878)	74480..75805	+	1326	WP_000969590.1	Y-family DNA polymerase	-
MKI60_RS14475 (MKI60_002879)	75798..76148	+	351	WP_000568378.1	hypothetical protein	-
MKI60_RS14480 (MKI60_002880)	76145..76324	+	180	WP_001021554.1	hypothetical protein	-
MKI60_RS14485 (MKI60_002881)	76460..76750	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / tet(S) / aac(6')-aph(2'') / VanHAX	-	1..78567	78567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T236519 WP_000588503.1 NZ_CP092545:71533-71790 [Staphylococcus aureus]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T236519 NZ_LR595848:1068895-1069149 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT236519 WP_000388479.1 NZ_CP092545:71271-71540 [Staphylococcus aureus]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT236519 NZ_LR595848:1068651-1068893 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7