Detailed information of TA system TA236513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102713..2103242
• Replicon: chromosome
• Accession: NZ_CP092544
• Organism: Staphylococcus aureus strain VMRSA-WC102

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI60_RS10440
• Protein ID: WP_000621175.1
• Coordinates: 2102713..2103075 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI60_RS10445
• Protein ID: WP_000948331.1
• Coordinates: 2103072..2103242 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI60_RS10420 (MKI60_002067)	2099691..2100461	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI60_RS10425 (MKI60_002068)	2100436..2100915	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI60_RS10430 (MKI60_002069)	2100917..2101243	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI60_RS10435 (MKI60_002070)	2101362..2102363	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI60_RS10440 (MKI60_002071)	2102713..2103075	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI60_RS10445 (MKI60_002072)	2103072..2103242	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI60_RS10450 (MKI60_002073)	2103327..2104475	-	1149	WP_001281154.1	alanine racemase	-
MKI60_RS10455 (MKI60_002074)	2104541..2104900	-	360	WP_000581197.1	holo-ACP synthase	-
MKI60_RS10460 (MKI60_002075)	2104904..2105395	-	492	WP_001205912.1	PH domain-containing protein	-
MKI60_RS10465 (MKI60_002076)	2105382..2106965	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI60_RS10470 (MKI60_002077)	2106958..2107437	-	480	WP_001287087.1	hypothetical protein	-
MKI60_RS10475 (MKI60_002078)	2107645..2108205	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236513 WP_000621175.1 NZ_CP092544:c2103075-2102713 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236513 NZ_LR595691:4469512-4469685 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236513 WP_000948331.1 NZ_CP092544:c2103242-2103072 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236513 NZ_LR595691:4469188-4469484 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1