Detailed information of TA system TA236512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_XRE(antitoxin)
• Location: 1962845..1963642
• Replicon: chromosome
• Accession: NZ_CP092544
• Organism: Staphylococcus aureus strain VMRSA-WC102

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MKI60_RS09685
• Protein ID: WP_000525008.1
• Coordinates: 1963181..1963642 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C2LD36
• Locus tag: MKI60_RS09680
• Protein ID: WP_001260487.1
• Coordinates: 1962845..1963168 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI60_RS09615 (MKI60_001908)	1958162..1958695	-	534	WP_001004326.1	host-nuclease inhibitor Gam family protein	-
MKI60_RS09620 (MKI60_001909)	1958688..1958939	-	252	WP_000683290.1	hypothetical protein	-
MKI60_RS09625 (MKI60_001910)	1958949..1959209	-	261	WP_000291481.1	DUF1108 family protein	-
MKI60_RS09630 (MKI60_001911)	1959214..1959516	-	303	WP_000163430.1	DUF2482 family protein	-
MKI60_RS09635 (MKI60_001912)	1959609..1959770	-	162	WP_000048124.1	DUF1270 family protein	-
MKI60_RS09640 (MKI60_001913)	1959763..1959978	-	216	WP_000977384.1	hypothetical protein	-
MKI60_RS09645 (MKI60_001914)	1959992..1960441	-	450	WP_000993183.1	hypothetical protein	-
MKI60_RS09650 (MKI60_001915)	1960481..1960705	-	225	WP_000187184.1	hypothetical protein	-
MKI60_RS09655 (MKI60_001916)	1960706..1961497	-	792	WP_001157019.1	phage antirepressor KilAC domain-containing protein	-
MKI60_RS09660 (MKI60_001917)	1961554..1961793	+	240	WP_000438628.1	hypothetical protein	-
MKI60_RS09665 (MKI60_001918)	1961783..1961962	-	180	WP_000393082.1	hypothetical protein	-
MKI60_RS09670 (MKI60_001919)	1961977..1962420	-	444	WP_000435356.1	hypothetical protein	-
MKI60_RS09675 (MKI60_001920)	1962433..1962681	-	249	WP_000272859.1	helix-turn-helix transcriptional regulator	-
MKI60_RS09680 (MKI60_001921)	1962845..1963168	+	324	WP_001260487.1	helix-turn-helix transcriptional regulator	Antitoxin
MKI60_RS09685 (MKI60_001922)	1963181..1963642	+	462	WP_000525008.1	hypothetical protein	Toxin
MKI60_RS09690 (MKI60_001923)	1963826..1964347	+	522	WP_001102444.1	hypothetical protein	-
MKI60_RS09695 (MKI60_001924)	1964340..1964810	+	471	WP_000183249.1	hypothetical protein	-
MKI60_RS09700 (MKI60_001925)	1964880..1965926	+	1047	WP_001145732.1	tyrosine-type recombinase/integrase	-
MKI60_RS09705 (MKI60_001926)	1965971..1967116	+	1146	WP_001245449.1	radical SAM/CxCxxxxC motif protein YfkAB	-
MKI60_RS09710 (MKI60_001927)	1967376..1967906	-	531	WP_000184383.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1921473..1965926	44453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18034.35 Da
• Isoelectric Point: 4.6899

>T236512 WP_000525008.1 NZ_CP092544:1963181-1963642 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSNFNNRKFENYAR
RHGFISAVPLREIVEAHNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T236512 NZ_LR595691:3914712-3915089 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12431.09 Da
• Isoelectric Point: 9.3811

>AT236512 WP_001260487.1 NZ_CP092544:1962845-1963168 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 324 bp

>AT236512 NZ_LR595691:3914288-3914665 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0C2LD36