Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2472618..2473141 Replicon chromosome
Accession NZ_CP092542
Organism Staphylococcus aureus strain VRMSSA-WC113

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag MKI55_RS12340 Protein ID WP_000074049.1
Coordinates 2472618..2472884 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag MKI55_RS12345 Protein ID WP_000587616.1
Coordinates 2472884..2473141 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI55_RS12320 (MKI55_002446) 2467925..2469112 - 1188 WP_000026189.1 MFS transporter -
MKI55_RS12325 (MKI55_002447) 2469508..2470284 - 777 WP_000072149.1 iron export ABC transporter permease subunit FetB -
MKI55_RS12330 (MKI55_002448) 2470277..2470939 - 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
MKI55_RS12335 (MKI55_002449) 2471187..2472263 - 1077 WP_001076673.1 M42 family metallopeptidase -
MKI55_RS12340 (MKI55_002450) 2472618..2472884 - 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
MKI55_RS12345 (MKI55_002451) 2472884..2473141 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MKI55_RS12350 (MKI55_002452) 2473500..2473955 + 456 WP_000732076.1 DUF1307 domain-containing protein -
MKI55_RS12355 (MKI55_002453) 2474123..2475688 - 1566 WP_000143492.1 FMN-binding glutamate synthase family protein -
MKI55_RS12360 (MKI55_002454) 2475883..2476632 + 750 WP_000072461.1 CPBP family lipoprotein N-acylation protein LnsB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T236506 WP_000074049.1 NZ_CP092542:c2472884-2472618 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T236506 NZ_LR595691:c2852763-2852452 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT236506 WP_000587616.1 NZ_CP092542:c2473141-2472884 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT236506 NZ_LR595691:c2852455-2852036 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References