Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2097252..2097766 Replicon chromosome
Accession NZ_CP092542
Organism Staphylococcus aureus strain VRMSSA-WC113

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI55_RS10395 Protein ID WP_000581792.1
Coordinates 2097252..2097461 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI55_RS10400 Protein ID WP_000138932.1
Coordinates 2097473..2097766 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI55_RS10375 (MKI55_002060) 2092760..2094118 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI55_RS10380 (MKI55_002061) 2094133..2095203 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI55_RS10385 (MKI55_002062) 2095521..2096723 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI55_RS10390 (MKI55_002063) 2096987..2097124 - 138 WP_000828354.1 Lmo0850 family protein -
MKI55_RS10395 (MKI55_002064) 2097252..2097461 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI55_RS10400 (MKI55_002065) 2097473..2097766 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI55_RS10405 (MKI55_002066) 2097932..2099425 + 1494 WP_000571554.1 cardiolipin synthase -
MKI55_RS10410 (MKI55_002067) 2099453..2100100 + 648 WP_001187633.1 HD domain-containing protein -
MKI55_RS10415 (MKI55_002068) 2101013..2101885 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI55_RS10420 (MKI55_002069) 2101971..2102612 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236502 WP_000581792.1 NZ_CP092542:c2097461-2097252 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236502 NZ_LR595691:c1946429-1946326 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236502 WP_000138932.1 NZ_CP092542:c2097766-2097473 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236502 NZ_LR595691:1946288-1946559 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References