Detailed information of TA system TA236501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2075993..2076522
• Replicon: chromosome
• Accession: NZ_CP092542
• Organism: Staphylococcus aureus strain VRMSSA-WC113

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI55_RS10295
• Protein ID: WP_000621175.1
• Coordinates: 2075993..2076355 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI55_RS10300
• Protein ID: WP_000948331.1
• Coordinates: 2076352..2076522 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI55_RS10275 (MKI55_002041)	2072971..2073741	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI55_RS10280 (MKI55_002042)	2073716..2074195	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI55_RS10285 (MKI55_002043)	2074197..2074523	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI55_RS10290 (MKI55_002044)	2074642..2075643	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI55_RS10295 (MKI55_002045)	2075993..2076355	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI55_RS10300 (MKI55_002046)	2076352..2076522	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI55_RS10305 (MKI55_002047)	2076607..2077755	-	1149	WP_001281154.1	alanine racemase	-
MKI55_RS10310 (MKI55_002048)	2077821..2078180	-	360	WP_000581197.1	holo-ACP synthase	-
MKI55_RS10315 (MKI55_002049)	2078184..2078675	-	492	WP_001205912.1	PH domain-containing protein	-
MKI55_RS10320 (MKI55_002050)	2078662..2080245	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI55_RS10325 (MKI55_002051)	2080238..2080717	-	480	WP_001287087.1	hypothetical protein	-
MKI55_RS10330 (MKI55_002052)	2080925..2081485	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236501 WP_000621175.1 NZ_CP092542:c2076355-2075993 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236501 NZ_LR595691:c1621691-1620369 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236501 WP_000948331.1 NZ_CP092542:c2076522-2076352 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236501 NZ_LR595691:c1621957-1621691 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1