Detailed information of TA system TA023650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3460242..3460758
• Replicon: chromosome
• Accession: NC_012214
• Organism: Erwinia pyrifoliae Ep1/96

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2T7S6
• Locus tag: EPC_RS16050
• Protein ID: WP_012669379.1
• Coordinates: 3460474..3460758 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2T7S5
• Locus tag: EPC_RS16045
• Protein ID: WP_012669378.1
• Coordinates: 3460242..3460484 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPC_RS16030	3455504..3455905	-	402	WP_012669375.1	helix-turn-helix domain-containing protein	-
EPC_RS16035	3456018..3458711	+	2694	WP_012669376.1	toprim domain-containing protein	-
EPC_RS16040	3458926..3459981	+	1056	WP_012669377.1	site-specific tyrosine recombinase XerC	-
EPC_RS16045	3460242..3460484	+	243	WP_012669378.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EPC_RS16050	3460474..3460758	+	285	WP_012669379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EPC_RS20510	3461260..3461436	-	177	WP_157861020.1	hypothetical protein	-
EPC_RS20040	3461571..3461753	+	183	WP_081441632.1	hypothetical protein	-
EPC_RS20045	3461794..3462117	+	324	Protein_3104	hypothetical protein	-
EPC_RS16060	3462132..3462344	+	213	WP_012669382.1	hypothetical protein	-
EPC_RS16065	3462392..3462694	-	303	WP_012669383.1	type I toxin-antitoxin system SymE family toxin	-
EPC_RS16070	3462773..3463147	-	375	WP_012669384.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3442437..3484446	42009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11123.03 Da
• Isoelectric Point: 10.3769

>T23650 WP_012669379.1 NC_012214:3460474-3460758 [Erwinia pyrifoliae Ep1/96]
MSYELVFDPRALKEWKKLGATVREQFKKKLAEVLVNPRVESARLREYPDCYKIKLKSSGYRLVYQVQDEQLVVFVVATGK
RERLQVYRDAGKRL

Nucleotide

• Length: 285 bp

>T23650 NC_012214:3460474-3460758 [Erwinia pyrifoliae Ep1/96]
ATGAGCTATGAACTGGTCTTCGATCCCAGAGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAGCAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCCGAGGGTGGAATCTGCCAGGTTGAGAGAGTACCCGGACTGCTACAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTTGTCTTCGTTGTGGCGACAGGAAAA
AGAGAAAGGTTACAGGTGTATCGTGACGCAGGAAAGCGCCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9070.45 Da
• Isoelectric Point: 4.6310

>AT23650 WP_012669378.1 NC_012214:3460242-3460484 [Erwinia pyrifoliae Ep1/96]
MATLNVRLDDKLKSKAYAVLDELDVSPTEAVRLLFQYIAENERLPLKTLTVSDEEDALLKTVRERLASPQKGIRVTLDEL

Nucleotide

• Length: 243 bp

>AT23650 NC_012214:3460242-3460484 [Erwinia pyrifoliae Ep1/96]
ATGGCAACATTAAACGTGCGGCTGGATGACAAGCTGAAAAGCAAAGCTTACGCAGTCCTGGATGAACTGGATGTATCACC
AACAGAGGCGGTCCGGCTGCTGTTCCAGTATATAGCGGAAAATGAACGTCTGCCGCTGAAAACGCTGACGGTCAGCGATG
AAGAGGACGCGCTGCTCAAAACGGTGCGCGAAAGGCTGGCATCGCCACAGAAGGGAATAAGGGTCACGCTGGATGAGCTA
TGA

Structures

Toxin

• AlphaFold DB: D2T7S6

Antitoxin

• AlphaFold DB: D2T7S5