Detailed information of TA system TA236496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 825570..826047
• Replicon: chromosome
• Accession: NZ_CP092542
• Organism: Staphylococcus aureus strain VRMSSA-WC113

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MKI55_RS03955
• Protein ID: WP_000052076.1
• Coordinates: 825570..825758 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MKI55_RS03960
• Protein ID: WP_001059690.1
• Coordinates: 825787..826047 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI55_RS03925 (MKI55_000777)	821564..822085	+	522	WP_000824482.1	hypothetical protein	-
MKI55_RS03930 (MKI55_000778)	822426..823112	+	687	WP_000141557.1	thermonuclease family protein	-
MKI55_RS03935 (MKI55_000779)	823469..823669	+	201	WP_001059082.1	cold-shock protein	-
MKI55_RS03940 (MKI55_000780)	824161..824379	-	219	WP_000005886.1	hypothetical protein	-
MKI55_RS03945 (MKI55_000781)	824441..824725	-	285	WP_001004399.1	hypothetical protein	-
MKI55_RS03950 (MKI55_000782)	824813..825382	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
MKI55_RS03955 (MKI55_000783)	825570..825758	-	189	WP_000052076.1	hypothetical protein	Toxin
MKI55_RS03960 (MKI55_000784)	825787..826047	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MKI55_RS03965 (MKI55_000785)	826388..826591	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MKI55_RS03970 (MKI55_000786)	826588..826824	-	237	WP_000349581.1	hypothetical protein	-
MKI55_RS03975 (MKI55_000787)	827049..827630	+	582	WP_001140693.1	histidine phosphatase family protein	-
MKI55_RS03980 (MKI55_000788)	827703..828320	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
MKI55_RS03985 (MKI55_000789)	828475..828969	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MKI55_RS03990 (MKI55_000790)	829368..829790	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
MKI55_RS03995 (MKI55_000791)	829938..830654	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T236496 WP_000052076.1 NZ_CP092542:c825758-825570 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T236496 NZ_LR595691:c453473-453255 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT236496 WP_001059690.1 NZ_CP092542:c826047-825787 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT236496 NZ_LR595691:c453873-453499 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56