Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2123990..2124504 Replicon chromosome
Accession NZ_CP092540
Organism Staphylococcus aureus strain VMRSA-WC121

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI67_RS10545 Protein ID WP_000581792.1
Coordinates 2123990..2124199 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI67_RS10550 Protein ID WP_000138932.1
Coordinates 2124211..2124504 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI67_RS10525 (MKI67_002086) 2119498..2120856 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI67_RS10530 (MKI67_002087) 2120871..2121941 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI67_RS10535 (MKI67_002088) 2122259..2123461 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI67_RS10540 (MKI67_002089) 2123725..2123862 - 138 WP_000828354.1 Lmo0850 family protein -
MKI67_RS10545 (MKI67_002090) 2123990..2124199 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI67_RS10550 (MKI67_002091) 2124211..2124504 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI67_RS10555 (MKI67_002092) 2124670..2126163 + 1494 WP_000571554.1 cardiolipin synthase -
MKI67_RS10560 (MKI67_002093) 2126191..2126838 + 648 WP_001187633.1 HD domain-containing protein -
MKI67_RS10565 (MKI67_002094) 2127751..2128623 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI67_RS10570 (MKI67_002095) 2128709..2129350 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236490 WP_000581792.1 NZ_CP092540:c2124199-2123990 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236490 NZ_LR594690:277898-278239 [Variovorax sp. WDL1]
GTGACGGGTGCGTACGTCCCTGGCCGTGGCGACGTTGTCTGGTTAGACTTCGACCCCCAAGCGGGGCATGAACAGGCGGG
TCGCCGTCCCGCGCTGGTGCTTTCACCGGCGACGTACAACGGAAAGAGCGGTTTGATGCTCTGCTGCCCGGTCACAAACC
AGGCGAAGGGCTATCCCTTCGAAGTGCCGGTCACGCCGTCCTCCGGCAAGGTCACTGGAGTTGCCTTGACTGACCAAGTG
CGATGCCTCGATTGGCGCTCTCGCAATGCCTCGAAGTTCGCTTCAGTCACGACGAAGTGCACACTGGACGTGAGCGCGAA
AGTGAAGCTTCTACTGCCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236490 WP_000138932.1 NZ_CP092540:c2124504-2124211 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236490 NZ_LR594690:277668-277901 [Variovorax sp. WDL1]
ATGCGTGTTGAAGTTCAAAAGTGGGGCAACAGCGCCGCGGTGCGTGTCCCTGCTCCGGCGCTGAAAGACGCCGGTATGCA
GGTCGGCCAGAGCCTTGAGCTGCGGGTTGAAGGGGGCAAGCTGGTCATCGAGCCGGCGACAGAAAGCCTCGAAGACCTGC
TGGCGCAGATGACCCCGGAAAACCAGCATGGTTTGGCACTGGAAGGGCCGGCGGTAGGGGCTGAGGCTTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References