Detailed information of TA system TA236489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102731..2103260
• Replicon: chromosome
• Accession: NZ_CP092540
• Organism: Staphylococcus aureus strain VMRSA-WC121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI67_RS10445
• Protein ID: WP_000621175.1
• Coordinates: 2102731..2103093 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI67_RS10450
• Protein ID: WP_000948331.1
• Coordinates: 2103090..2103260 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI67_RS10425 (MKI67_002067)	2099709..2100479	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI67_RS10430 (MKI67_002068)	2100454..2100933	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI67_RS10435 (MKI67_002069)	2100935..2101261	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI67_RS10440 (MKI67_002070)	2101380..2102381	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI67_RS10445 (MKI67_002071)	2102731..2103093	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI67_RS10450 (MKI67_002072)	2103090..2103260	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI67_RS10455 (MKI67_002073)	2103345..2104493	-	1149	WP_001281154.1	alanine racemase	-
MKI67_RS10460 (MKI67_002074)	2104559..2104918	-	360	WP_000581197.1	holo-ACP synthase	-
MKI67_RS10465 (MKI67_002075)	2104922..2105413	-	492	WP_001205912.1	PH domain-containing protein	-
MKI67_RS10470 (MKI67_002076)	2105400..2106983	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI67_RS10475 (MKI67_002077)	2106976..2107455	-	480	WP_001287087.1	hypothetical protein	-
MKI67_RS10480 (MKI67_002078)	2107663..2108223	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236489 WP_000621175.1 NZ_CP092540:c2103093-2102731 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236489 NZ_LR594689:6465979-6466851 [Variovorax sp. WDL1]
ATGAGCGATGCAGCCGTGATCGACGCCCAACCCCTGCCGGCTTCCGAGCTGGCCCGCCTCGATGCGCGCTTCCCGGCGCG
CAGCGTGCCGGTACAGGGCGGGTCCTTGGTGTCGGTGCGCGAATGCGGCGCGGGACGAGCGATCGTGTGCCTGCACGGCA
TCGGCTCGGGCTCGGCCTCGTGGCTGGACACGGCGCTTCTGCTCGAAGGCGAGGCGCGGCTGATCGCGTGGGATGCACCG
GGCTACGGCGAATCGACGCCGCTCGCAGCGCAGGCGCCCACCTCGCGCGACTACGCGCAGCGCCTGCATGGCCTGCTCGA
TGCGCTGGAGATTGCATCGTGCGTGCTGGTCGGCCATTCGCTCGGCGCGCTGACGGCTGCAGCTGCGGCGCGCCCCGGTT
CGCCGCTGGCGTCGCGCATCTCGAAGCTGCTGCTGATCAGCCCGGCAGCGGGCTACGGTGCGGCGAGCCGCGCGCAGTCG
CAGGGCCGCGTGCGTGCAGAACGCCTGGGCACGCTCGATGAACTCGGCATCGCCGGCATGGCCGCGAAGCGCTCGGGACG
GCTGGTCTCGGCGCATGCGAGCGATACCGCGCGCCAGTGGGTGCGCTGGAACATGGCGCGCCTGCACGACACAGGCTACC
GCCAGGCCGTGGAGCTGCTGTGCGGCGCCGACCTGCTGGCCGACCTGCCGCCAGCCATGCCGGTGCGCGTGGCCTGCGGC
GCGCTGGACGTGGTCACCACGCCCGAGGCCTGCGCCGAGGTGGCAAGGCAGTGCGGCGTGCCGCTCGAACTGGTGCCCGA
GGCGGGCCACGCGAGCTACGTCGAGCGGCCCGCCGCAGTGGCCGCGCTGCTGCGCGATGCCCTTGCAGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236489 WP_000948331.1 NZ_CP092540:c2103260-2103090 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236489 NZ_LR594689:6466876-6467703 [Variovorax sp. WDL1]
ATGAGCAACGACCTGATGGAAGAGGGCGCCGACCGCTACAACGTGCCGGCCCTGGAACGCGGCCTTCGCATGCTGTGCGA
GTTCAGCCGCGAGAGCCGCACCCTGTCCGCGCCCGAGCTCGCGCGGCGCTTCGACCTGCCGCGCTCGACCGTGTTCCGCC
TGCTCAGCACGCTGGAGAACATGGGCTTCCTGGAGCGCGCCGAGGGCGGCCGCGACTACCGCCTGGGCCTGGCCGTGCTG
CGCCTGGGCTTCGAGTACCTGGCTTCGCTGGAGCTCACGCAGCTGGGCACGCCGCTGCTCAACCGCCTGTGCGAGGAGCT
GCGCACGCCCTGCAACCTGGTGGTGCGCGACGGCCGCTCCATCGTCTACGTGGCGAAGGTCGCGCCGCCCACGCCGTTCG
CGAGCTCGGTCAGCGTCGGCACGCGCCTGCCGGCCCACGCGACCGTGCTGGGCCGCATCCTGCTGGAAGACCTGACGCTG
CCGCAACTGCGCGCGCTCTACCCGGAGGACAAGCTGGAGACCTTCTCGCCGAGCACGCCCAAGACCGTCAACGAGCTCTT
CGACATGGTCCAGGCCGACCGCGAGCGCGGCTACGTGCTGGGCGAGGGCTTCTTCGAATCGAACATCTCCACCATTGCCG
CGCCGGTGCGCGACCACAGCGGCCACATCGCCGCCGCATTGGGCGCAACCATCACCTCCGGCCACATCGACGAGAACCGC
ATGGACGACATGGTGCGCCGCGTGCGCGAAACGGCCGACGAGATCTCGGGCCTCCTGAACTACGCGCCCGCCAGCAAGGC
CAAGGTGGTCCCGCTGCGCAGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1