Detailed information of TA system TA023648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3000680..3001214
• Replicon: chromosome
• Accession: NC_012214
• Organism: Erwinia pyrifoliae Ep1/96

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2T5B9
• Locus tag: EPC_RS13940
• Protein ID: WP_012668982.1
• Coordinates: 3000680..3000967 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2T5C0
• Locus tag: EPC_RS13945
• Protein ID: WP_012668983.1
• Coordinates: 3000972..3001214 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPC_RS13915	2995995..2997194	-	1200	WP_012668976.1	S-methyl-5-thioribose kinase	-
EPC_RS13920	2997309..2998340	+	1032	WP_012668977.1	S-methyl-5-thioribose-1-phosphate isomerase	-
EPC_RS13925	2998392..2998934	-	543	WP_012668978.1	acireductone dioxygenase	-
EPC_RS13930	2998931..2999620	-	690	WP_012668979.1	acireductone synthase	-
EPC_RS13935	2999617..3000231	-	615	WP_012668980.1	methylthioribulose 1-phosphate dehydratase	-
EPC_RS13940	3000680..3000967	-	288	WP_012668982.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EPC_RS13945	3000972..3001214	-	243	WP_012668983.1	plasmid stabilization protein	Antitoxin
EPC_RS13950	3001636..3002796	+	1161	WP_012668984.1	pyridoxal phosphate-dependent aminotransferase	-
EPC_RS13955	3002784..3003557	+	774	WP_041474033.1	amidohydrolase	-
EPC_RS13960	3003541..3004866	-	1326	WP_012668986.1	purine permease	-
EPC_RS13965	3004899..3005234	-	336	WP_012668987.1	hydroxyisourate hydrolase	-
EPC_RS13970	3005231..3005725	-	495	WP_012668988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10939.91 Da
• Isoelectric Point: 10.7969

>T23648 WP_012668982.1 NC_012214:c3000967-3000680 [Erwinia pyrifoliae Ep1/96]
MIYKVKIRAEALKEWHGLDSAVREQFSKKLKKLRENPHVPSARLTGIAGCYKIKLRSSGFRLVYQVIDEELIIAVVAVGK
RERSQAYKLASERLR

Nucleotide

• Length: 288 bp

>T23648 NC_012214:c3000967-3000680 [Erwinia pyrifoliae Ep1/96]
TTGATTTATAAGGTTAAAATCAGGGCAGAAGCCCTGAAAGAATGGCATGGTTTGGACAGTGCCGTTCGGGAGCAATTTAG
TAAAAAACTTAAAAAATTGCGTGAAAATCCGCATGTACCTTCAGCCAGGCTAACGGGAATAGCGGGATGTTACAAAATAA
AACTCAGGTCTTCCGGCTTTCGGCTGGTATATCAGGTTATTGATGAAGAGCTGATTATAGCTGTGGTAGCTGTTGGAAAA
AGAGAGCGCAGCCAGGCATATAAACTTGCAAGCGAAAGGTTGCGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8820.18 Da
• Isoelectric Point: 4.0233

>AT23648 WP_012668983.1 NC_012214:c3001214-3000972 [Erwinia pyrifoliae Ep1/96]
MANLILSDTSASVSELKKNPMATVDAGCGFPVAILNRNQPAFYCIPAPLYERMLEVIDDQELVTLVKARENQPLIDLDLD

Nucleotide

• Length: 243 bp

>AT23648 NC_012214:c3001214-3000972 [Erwinia pyrifoliae Ep1/96]
ATGGCAAATCTCATTCTTAGTGATACCAGTGCAAGCGTAAGCGAGCTTAAAAAGAATCCGATGGCTACAGTTGATGCCGG
TTGCGGCTTTCCTGTAGCGATTCTCAACCGCAATCAGCCCGCTTTCTACTGCATCCCTGCACCACTCTATGAGCGCATGC
TGGAAGTGATAGACGATCAGGAACTGGTCACGCTGGTTAAAGCGCGTGAAAATCAACCTCTGATAGATCTCGATCTGGAC
TAA

Structures

Toxin

• AlphaFold DB: D2T5B9

Antitoxin

• AlphaFold DB: D2T5C0