Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2123578..2124092 Replicon chromosome
Accession NZ_CP092538
Organism Staphylococcus aureus strain VMRSA-WC123

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI57_RS10540 Protein ID WP_000581792.1
Coordinates 2123578..2123787 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI57_RS10545 Protein ID WP_000138932.1
Coordinates 2123799..2124092 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI57_RS10520 (MKI57_002085) 2119086..2120444 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI57_RS10525 (MKI57_002086) 2120459..2121529 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI57_RS10530 (MKI57_002087) 2121847..2123049 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI57_RS10535 (MKI57_002088) 2123313..2123450 - 138 WP_000828354.1 Lmo0850 family protein -
MKI57_RS10540 (MKI57_002089) 2123578..2123787 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI57_RS10545 (MKI57_002090) 2123799..2124092 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI57_RS10550 (MKI57_002091) 2124258..2125751 + 1494 WP_000571554.1 cardiolipin synthase -
MKI57_RS10555 (MKI57_002092) 2125779..2126426 + 648 WP_001187633.1 HD domain-containing protein -
MKI57_RS10560 (MKI57_002093) 2127339..2128211 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI57_RS10565 (MKI57_002094) 2128297..2128938 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236478 WP_000581792.1 NZ_CP092538:c2123787-2123578 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236478 NZ_LR594674:36689-37297 [Variovorax sp. PBL-E5]
ATGAAATACGCCGGGGATCGCGGCGATCCTTACCTGGACAGCGAAACGGGTGTTCTCCGCAATCTCCTTGGAATCAAGGA
ACAGGGTGGACTCGATAAAGCCGAATCCACCCTTTCCTTTTTGCGGGCCAGCGAATTGCGCGAGCAGCCCGTTAAAGGCA
AATTCGATTTAGCGCACCTGCAGCGAATTCACAAGCGCCTTTTTGGCGACGTGTACGACTGGGCGGGCCAAATCCGCCAG
GTCGAAATCTCGAAAGGCAGCACCATGTTTGCCCGGCAGGTTGCGATCCAGAGCGCGGCGCAGCAGCTCTTTGGGCAGCT
TGCCAAGGAACAGCTCTTGCGCGGCCTCGATGCCGACGAGTTCAGCAAGCGGGCCGGCCACTATCTCGGGGAAATCAACG
TGCTTCATCCCTTCCGTGAAGGGAACGGCAGGACGCAGCGTGAGTTCATCGGGCAACTGGCCCAGCAGGCGGGCCACAGG
ATCGACTGGAGCGGGGTCAGTCAGGCCAGCATGACCCAGGCGTCAATCGAGGCCTACAACGGCGACTCAAGCGGTATGGC
TGGCCTTATTCGCGCTGGCATGCCAGACCAGCTTTTTTTTAACCCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236478 WP_000138932.1 NZ_CP092538:c2124092-2123799 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236478 NZ_LR594674:36504-36692 [Variovorax sp. PBL-E5]
ATGATCGCAGAGCAGGAACAGACCGAGCGCCGCGCCGTGGTGCAAAGCGCCCTTGCGAGCCAGCGCATCGAGGGCTTAGA
GCCCGATGCGCAGGCCGTGGCCGACGCCGAGCGCTGGGCGCGTGGCGAGATGACCATCGGTGCCGCCGTGGACCAGTACA
AGGCGCGCATGCGGCTAGAAATGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References