Detailed information of TA system TA236477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102319..2102848
• Replicon: chromosome
• Accession: NZ_CP092538
• Organism: Staphylococcus aureus strain VMRSA-WC123

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI57_RS10440
• Protein ID: WP_000621175.1
• Coordinates: 2102319..2102681 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI57_RS10445
• Protein ID: WP_000948331.1
• Coordinates: 2102678..2102848 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI57_RS10420 (MKI57_002066)	2099297..2100067	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI57_RS10425 (MKI57_002067)	2100042..2100521	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI57_RS10430 (MKI57_002068)	2100523..2100849	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI57_RS10435 (MKI57_002069)	2100968..2101969	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI57_RS10440 (MKI57_002070)	2102319..2102681	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI57_RS10445 (MKI57_002071)	2102678..2102848	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI57_RS10450 (MKI57_002072)	2102933..2104081	-	1149	WP_001281154.1	alanine racemase	-
MKI57_RS10455 (MKI57_002073)	2104147..2104506	-	360	WP_000581197.1	holo-ACP synthase	-
MKI57_RS10460 (MKI57_002074)	2104510..2105001	-	492	WP_001205912.1	PH domain-containing protein	-
MKI57_RS10465 (MKI57_002075)	2104988..2106571	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI57_RS10470 (MKI57_002076)	2106564..2107043	-	480	WP_001287087.1	hypothetical protein	-
MKI57_RS10475 (MKI57_002077)	2107251..2107811	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236477 WP_000621175.1 NZ_CP092538:c2102681-2102319 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236477 NZ_LR594670:3305-3913 [Variovorax sp. SRS16]
ATGAAATACGCCGGGGATCGCGGCGATCCTTACCTGGACAGCGAAACGGGTGTTCTCCGCAATCTCCTTGGAATCAAGGA
ACAGGGTGGACTCGATAAAGCCGAATCCACCCTTTCCTTTTTGCGGGCCAGCGAATTGCGCGAGCAGCCCGTTAAAGGCA
AATTCGATTTAGCGCACCTGCAGCGAATTCACAAGCGCCTTTTTGGCGACGTGTACGACTGGGCGGGCCAAATCCGCCAG
GTCGAAATCTCGAAAGGCAGCACCATGTTTGCCCGGCAGGTTGCGATCCAGAGCGCGGCGCAGCAGCTCTTTGGGCAGCT
TGCCAAGGAACAGCTCTTGCGCGGCCTCGATGCCGACGAGTTCAGCAAGCGGGCCGGCCACTATCTCGGGGAAATCAACG
TGCTTCATCCCTTCCGTGAAGGGAACGGCAGGACGCAGCGTGAGTTCATCGGGCAACTGGCCCAGCAGGCGGGCCACAGG
ATCGACTGGAGCGGGGTCAGTCAGGCCAGCATGACCCAGGCGTCAATCGAGGCCTACAACGGCGACTCAAGCGGTATGGC
TGGCCTTATTCGCGCTGGCATGCCAGACCAGCTTTTTTTTAACCCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236477 WP_000948331.1 NZ_CP092538:c2102848-2102678 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236477 NZ_LR594670:3120-3308 [Variovorax sp. SRS16]
ATGATCGCAGAGCAGGAACAGACCGAGCGCCGCGCCGTGGTGCAAAGCGCCCTTGCGAGCCAGCGCATCGAGGGCTTAGA
GCCCGATGCGCAGGCCGTGGCCGACGCCGAGCGCTGGGCGCGTGGCGAGATGACCATCGGTGCCGCCGTGGACCAGTACA
AGGCGCGCATGCGGCTAGAAATGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1