Detailed information of TA system TA236475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1889404..1890180
• Replicon: chromosome
• Accession: NZ_CP092538
• Organism: Staphylococcus aureus strain VMRSA-WC123

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MKI57_RS09080
• Protein ID: WP_000031108.1
• Coordinates: 1889404..1889556 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MKI57_RS09085
• Protein ID: WP_001251224.1
• Coordinates: 1889581..1890180 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI57_RS09065 (MKI57_001798)	1885606..1886427	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MKI57_RS09070 (MKI57_001799)	1886880..1888265	-	1386	WP_000116228.1	class II fumarate hydratase	-
MKI57_RS09075 (MKI57_001800)	1888461..1888856	-	396	WP_000901023.1	hypothetical protein	-
MKI57_RS09080 (MKI57_001801)	1889404..1889556	-	153	WP_000031108.1	SAS053 family protein	Toxin
MKI57_RS09085 (MKI57_001802)	1889581..1890180	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MKI57_RS09090 (MKI57_001803)	1890339..1890809	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MKI57_RS09095 (MKI57_001804)	1890814..1891941	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
MKI57_RS09100 (MKI57_001805)	1892092..1892814	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MKI57_RS09105 (MKI57_001806)	1892807..1894264	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T236475 WP_000031108.1 NZ_CP092538:c1889556-1889404 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T236475 NZ_LR594668:c488812-488516 [Variovorax sp. SRS16]
ATGCCACGGTTGATCTGGTCGCCGCACGCGCTTAGCGACGTGCAGCGTCTTTACCGCTTCCTCTGCGGGAAGAACCCGGA
TGCTGCCCGACGCGCAGTCCGAACCATCCGCGACGGCATGATGGTTGTGGCGCAACAGCCAGGCATCGGGCGACCCATTG
CCGACATGGAGCCTGAATACAGGGAATGGCTTATCGACTTCGGTGCCGGTGGCTATGTGGCGAAGTACCGTTTGGATGGC
GAAACGGCGGTCGTCTTGGCGGTGCGGCACCAGCGGGAAGTCGGGTATGAGGAATGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT236475 WP_001251224.1 NZ_CP092538:c1890180-1889581 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT236475 NZ_LR594668:c489090-488800 [Variovorax sp. SRS16]
ATGAATACTTCAGCTCTTCGTCCCGTCACGGTCAAGATCGAACCCTCGGTCAAACTGCGAGTGCAGCGTTTGGCAAAGGC
ACGCCACCGTACGCCTCATTGGGTGATGCACGAGGCCATTCTCCAGTTTGTCGAACGTGAGGAAAAGCGAGAAGCATTTC
GCCAGGACGCGATCAGGGCATGGGACGAATACCGACTGACCGGCTTGCACGTCACAGCGGCGGAGGCAGATGCCTGGATG
GCCAAGCTCGAGACCGGGCAGGACGTTGAGCCGCCTGAATGCCACGGTTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4