Detailed information of TA system TA236471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 161020..161690
• Replicon: plasmid unnamed1
• Accession: NZ_CP092537
• Organism: Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: UPHL_RS26985
• Protein ID: WP_004213072.1
• Coordinates: 161020..161463 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: UPHL_RS26990
• Protein ID: WP_004213073.1
• Coordinates: 161460..161690 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UPHL_RS26950 (UPHL_005369)	156430..156705	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
UPHL_RS26955 (UPHL_005370)	156768..157259	+	492	WP_011251327.1	DM13 domain-containing protein	-
UPHL_RS26960 (UPHL_005371)	157308..158228	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
UPHL_RS26965 (UPHL_005372)	158319..158722	+	404	Protein_174	GAF domain-containing protein	-
UPHL_RS26970 (UPHL_005373)	159240..159876	-	637	Protein_175	mucoid phenotype regulator RmpA2	-
UPHL_RS26975 (UPHL_005374)	160293..160597	+	305	Protein_176	transposase	-
UPHL_RS26980	160620..160871	-	252	WP_186987481.1	hypothetical protein	-
UPHL_RS26985 (UPHL_005376)	161020..161463	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
UPHL_RS26990 (UPHL_005377)	161460..161690	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
UPHL_RS26995 (UPHL_005378)	162298..163431	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
UPHL_RS27000 (UPHL_005379)	163447..163740	+	294	WP_004213076.1	hypothetical protein	-
UPHL_RS27005 (UPHL_005380)	163730..163936	-	207	WP_004213077.1	hypothetical protein	-
UPHL_RS27010 (UPHL_005381)	164288..164578	+	291	WP_004213078.1	hypothetical protein	-
UPHL_RS27015 (UPHL_005382)	164568..165467	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..235474	235474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T236471 WP_004213072.1 NZ_CP092537:c161463-161020 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T236471 NZ_LR594666:2281523-2282386 [Variovorax sp. SRS16]
ATGAGCGACGCCGCCATGATCTCCGCGCTGCCGCCGTCCGAAGTGGCACGCCTCGACGCGCGTTTTCCGGCGCGCCGTGT
GCCGGTGCGTGGCGGCGTGGTTTCCGTGCGCGCGTGCGGTGCCGGCCCGGTGATCGTGTGTCTGCACGGCATCGGTTCGG
GCGCGGCCTCGTGGCTCGACACGGCGTTGCTGCTCGAGCCGCATGCGCACCTGATCGCATGGGACGCGCCGGGCTACGGC
GAATCCACGCCGGTGGTGCCCGAAGTGCCGATGGCGGCGGACTATGCCGAGCGGCTGCATGCCTTGCTCGACGCGATGGA
CATCGAATCCTGCGTGCTGGTCGGACATTCGCTCGGCGCGCTCACCGCGGCTTTCGCGGCGCGCGCGGGTTCGCCGCTGG
CCGCGCGTATCGAGCGGCTGGTGCTCATCAGCCCGGCCGGTGGCTATGGCGCACCCGCCCGTGCGCAGGCCCGCCAGCGC
GTCCGCACGGAACGGCTCGACACGCTGGCCAGGCTCGGCGTCGCCGGCATGGCCACGCAACGCTCGGGCCGCCTGGTATC
GGACAACGCGAGCGAACTGGCCCGCCAATGGGTGCGCTGGAACATGGCGCGCCTGAACGACCACGGCTACCGCCAGGCCG
TCGAACTGCTGTGCGGCGGCGACCTGCAGGCCGACCTGCCGCCCGCCATGCCCGTCCGCGTGGCCTGCGGCGCGCTCGAT
GTCGTCACGACGCCTGCCGCCTGCGAAGAGGTGGCGATCCGATGCGGCGTCGCGCTCGAACTGCTGGACGGCGCGGGGCA
TGCCAGCTACGTGGAGCAGCCGGAGACCGTCGCCGCGATGTTGCGCTACGTGCTCGCGTCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT236471 WP_004213073.1 NZ_CP092537:c161690-161460 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT236471 NZ_LR594666:2282419-2283258 [Variovorax sp. SRS16]
ATGACCACCCTTGGCAATGACGTGATGGAAGAAGGCGCGGACCGCTACAACGTTCCGGCGCTCGAGCGCGGCCTGCGCGT
GCTGTGCGAGTTCAGCCGCGAGAACCGCACGCTGTCCGCGCCCGAACTCGCGCGCCGCTTCGATCTGCCGCGCTCGACCG
TGTTCCGCCTGCTCACGACGCTGGAAAGCATGGGCTTCCTGGAGCGCGCCGAAGGCGGCCGCGACTACCGGCTCGGCCTG
GCGGTGCTGCGCCTCGGCTTCGAATACCTGGCCTCGCTGGAGCTCACGCAGCTCGGCACGCCGCTGCTGCAGCGCCTGTG
CGAAGAGCTGCGCACGCCGTGCAACCTGGTGGTGCGCGACGGCCGCTCGATCGTCTACGTCGCCAAGGTCGCACCGCCCA
CGCCATTCGCCAGCTCGGTCACCGTCGGCACGCGGCTGCCCGCACACGCCACGGTGCTCGGCCGCATCCTGCTCGAAGAC
CTGAGCCTGCCGCAGCTGCGCGCGCTCTATCCCGAAGACAAGCTCGAGACCTTCTCGCCGAGTACGCCGAAGACGGTCAA
CGAACTGTTCGACATGGTCCAGAGCGACCGCGATCGCGGCTACGTGCTGGGCGAAGGCTTCTTCGAATCGAACATCTCCA
GCATCGCCGCGCCGGTGCGCGACCACAGCGGCCACATCGTGGCGGCGCTGGGCGCGACCATCACCTCGGGCCACATCGAG
GAAGCCCGCATGGACGAGATGGTGCAGCGTGTGCGCGAGACGGCCGACGCCATCTCGGGCCTGTTGAACTATTCGCCGGC
GGCCAAGGTGGTTGCGTTGCGCGGCGGCCATCGCGCCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620