Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 95509..96245 Replicon plasmid unnamed1
Accession NZ_CP092537
Organism Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag UPHL_RS26650 Protein ID WP_004098919.1
Coordinates 95509..95991 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag UPHL_RS26655 Protein ID WP_004213599.1
Coordinates 95979..96245 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UPHL_RS26630 (UPHL_005305) 91280..92626 + 1347 WP_011154555.1 dihydroorotase -
UPHL_RS26635 (UPHL_005306) 92690..93712 + 1023 WP_004214536.1 porphobilinogen synthase -
UPHL_RS26640 (UPHL_005307) 93737..93865 + 129 Protein_109 IS3 family transposase -
UPHL_RS26645 (UPHL_005308) 93861..95088 - 1228 Protein_110 IS3 family transposase -
UPHL_RS26650 (UPHL_005309) 95509..95991 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
UPHL_RS26655 (UPHL_005310) 95979..96245 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
UPHL_RS26660 (UPHL_005311) 96440..96670 - 231 WP_004213598.1 hypothetical protein -
UPHL_RS26665 (UPHL_005312) 96684..96887 - 204 WP_004213596.1 HHA domain-containing protein -
UPHL_RS26670 (UPHL_005313) 96948..97442 - 495 WP_004213594.1 hypothetical protein -
UPHL_RS26675 (UPHL_005314) 97484..100453 - 2970 WP_004213592.1 Tn3 family transposase -
UPHL_RS26680 (UPHL_005315) 100456..101013 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..235474 235474
- inside IScluster/Tn - - 93861..101013 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T236470 WP_004098919.1 NZ_CP092537:c95991-95509 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T236470 NZ_LR594665:3305-3913 [Variovorax sp. RA8]
ATGAAATACGCCGGGGATCGCGGCGATCCTTACCTGGACAGCGAAACGGGTGTTCTCCGCAATCTCCTTGGAATCAAGGA
ACAGGGTGGACTCGATAAAGCCGAATCCACCCTTTCCTTTTTGCGGGCCAGCGAATTGCGCGAGCAGCCCGTTAAAGGCA
AATTCGATTTAGCGCACCTGCAGCGAATTCACAAGCGCCTTTTTGGCGACGTGTACGACTGGGCGGGCCAAATCCGCCAG
GTCGAAATCTCGAAAGGCAGCACCATGTTTGCCCGGCAGGTTGCGATCCAGAGCGCGGCGCAGCAGCTCTTTGGGCAGCT
TGCCAAGGAACAGCTCTTGCGCGGCCTCGATGCCGACGAGTTCAGCAAGCGGGCCGGCCACTATCTCGGGGAAATCAACG
TGCTTCATCCCTTCCGTGAAGGGAACGGCAGGACGCAGCGTGAGTTCATCGGGCAACTGGCCCAGCAGGCGGGCCACAGG
ATCGACTGGAGCGGGGTCAGTCAGGCCAGCATGACCCAGGCGTCAATCGAGGCCTACAACGGCGACTCAAGCGGTATGGC
TGGCCTTATTCGCGCTGGCATGCCAGACCAGCTTTTTTTTAACCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT236470 WP_004213599.1 NZ_CP092537:c96245-95979 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT236470 NZ_LR594665:3120-3308 [Variovorax sp. RA8]
ATGATCGCAGAGCAGGAACAGACCGAGCGCCGCGCCGTGGTGCAAAGCGCCCTTGCGAGCCAGCGCATCGAGGGCTTAGA
GCCCGATGCGCAGGCCGTGGCCGACGCCGAGCGCTGGGCGCGTGGCGAGATGACCATCGGTGCCGCCGTGGACCAGTACA
AGGCGCGCATGCGGCTAGAAATGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References