Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5319240..5319865 Replicon chromosome
Accession NZ_CP092536
Organism Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag UPHL_RS25730 Protein ID WP_002882817.1
Coordinates 5319240..5319623 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag UPHL_RS25735 Protein ID WP_004150355.1
Coordinates 5319623..5319865 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UPHL_RS25715 (5316606) 5316606..5317508 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
UPHL_RS25720 (5317505) 5317505..5318140 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
UPHL_RS25725 (5318137) 5318137..5319066 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
UPHL_RS25730 (5319240) 5319240..5319623 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
UPHL_RS25735 (5319623) 5319623..5319865 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
UPHL_RS25740 (5320070) 5320070..5320987 + 918 WP_029884108.1 alpha/beta hydrolase -
UPHL_RS25745 (5321001) 5321001..5321942 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
UPHL_RS25750 (5321987) 5321987..5322424 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
UPHL_RS25755 (5322421) 5322421..5323281 - 861 WP_002882807.1 virulence factor BrkB family protein -
UPHL_RS25760 (5323275) 5323275..5323874 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T236468 WP_002882817.1 NZ_CP092536:c5319623-5319240 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T236468 NZ_LR594663:c250904-250566 [Variovorax sp. RA8]
ATGGTTCGGCGCTATGTGCCCGAAGCCGGGGACATCGTGTGGCTGCATTTTGACCCGCAAGCCGGTCACGAGCAGGCCGG
CCATCGCCCCGCGTTGGTGCTCAGCCCGTCGGCGTACAACGGAAAGACCGGCTTGATGCTTTGCTGCCCTTTGACTACAC
AAGTAAAGGGCTATCCATTCGAGGTCAAGATTGCTGCAGCCCGAGCAGGCGCGGCCCTGGCCGATCAAGTGAAGAGCCTT
GATTGGGTAGCCCGCAGAGCGAGCCGCAAAGGCAGGGCCTCTGCCGCGGAGCTAGCAGAGGTCAGGGCAAAGGCAATCGC
CCTCATCGGCCAGCCTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT236468 WP_004150355.1 NZ_CP092536:c5319865-5319623 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT236468 NZ_LR594663:c251146-250904 [Variovorax sp. RA8]
ATGCATGTAATGGTGAAAAAATGGGGCAATAGCGCGTCGGTGCGCATTCCCGCGGCGGTGATGGAGGCTGCGCGGCTGCA
TTTGGACGAAACCGTTGACGTGCGTGAAGAGGAAGGGCGGATCGTGATCGAGCCTGTACGGCCCGGCGAATTTGAACTCA
CAAGATTGCTGGCCCGGATCACCCCGGAAAACTTGCATGGTGAAGTTAGCTTCGGCCCTGCTCTTGGCAAAGAGTCGTTC
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References