Detailed information of TA system TA236467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4995034..4996715
• Replicon: chromosome
• Accession: NZ_CP092536
• Organism: Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: UPHL_RS24225
• Protein ID: WP_004146663.1
• Coordinates: 4995034..4995951 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: UPHL_RS24230
• Protein ID: WP_004177809.1
• Coordinates: 4995957..4996715 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UPHL_RS24205 (4990340)	4990340..4991860	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
UPHL_RS24210 (4991857)	4991857..4992855	+	999	WP_004872882.1	ribose ABC transporter permease	-
UPHL_RS24215 (4992886)	4992886..4993827	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
UPHL_RS24220 (4993902)	4993902..4994909	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
UPHL_RS24225 (4995034)	4995034..4995951	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
UPHL_RS24230 (4995957)	4995957..4996715	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
UPHL_RS24235 (4996801)	4996801..4997529	-	729	WP_004177810.1	response regulator transcription factor	-
UPHL_RS24240 (4997802)	4997802..4999949	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
UPHL_RS24245 (5000411)	5000411..5001097	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T236467 WP_004146663.1 NZ_CP092536:c4995951-4995034 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T236467 NZ_LR594663:c245937-245641 [Variovorax sp. RA8]
GTGAGGCTTGAGTGGTCGCCGTTGGCGATGGATGACCGGGAGCGGATTTTCGACTTCATCGAACGAGACGATCCGCGCGC
GGCGATTGCAGTGGACGAGCGCATCGCCACCCAAGTGCTGGTGCTGTTGCAGTTTCCAGAAGGCGGGCGACCAGGTCGCG
TCGAAGGGACGCGCGAACTGGTCGTTCGCCGTACGTCGTACATCGTGGCCTATCGGGTTGGCAAGGATTGCGTTCGCATC
CTGCGAATTCTTCACAGCGCGCAGTTGTGGCCCGGCGAACTGACAGACCTGCCGTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT236467 WP_004177809.1 NZ_CP092536:c4996715-4995957 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT236467 NZ_LR594663:c246242-245934 [Variovorax sp. RA8]
ATGGCCGCGAACGCATTGGTACAGACCCGCATCGACGCTGAGGTGAAGGAGCGCGCCACCGCAGTGCTCGACAACATCGG
CCTCACCGTCTCGGACGTGATGAGGATCGTTCTGACACGTGTGGCCAAGGAGGGCGCATTGCCGGCGGGTTTTACCGTTG
ACGCTGCGGCCCATGACGCCTGGTTCCGAGCCAAGGTGCAGGAGGCCTTGGATGATCCGCGCCCTGCCATCCCGCATGAA
AAAGTTAATGCGCATTTCGCCAAGCGCCGCGCCGCTGCACTGCTCAAAGCGGGTGAAGGTAAGGCGTGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05