Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4705747..4706557 Replicon chromosome
Accession NZ_CP092536
Organism Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag UPHL_RS22870 Protein ID WP_004178461.1
Coordinates 4705747..4706280 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag UPHL_RS22875 Protein ID WP_004222378.1
Coordinates 4706291..4706557 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UPHL_RS22865 (4704578) 4704578..4705699 + 1122 WP_002887282.1 cupin domain-containing protein -
UPHL_RS22870 (4705747) 4705747..4706280 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
UPHL_RS22875 (4706291) 4706291..4706557 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
UPHL_RS22880 (4706660) 4706660..4708093 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
UPHL_RS22885 (4708083) 4708083..4708766 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
UPHL_RS22890 (4708938) 4708938..4710323 + 1386 WP_285811751.1 efflux transporter outer membrane subunit -
UPHL_RS22895 (4710341) 4710341..4710685 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T236466 WP_004178461.1 NZ_CP092536:c4706280-4705747 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T236466 NZ_LR594663:138706-139080 [Variovorax sp. RA8]
ATGAGCGTGCTCGTCGACACCTCTGTTTGGATCGACCATTTCCGCAGCGGCAACGCCGCCCTCGCGCACCTGGTCGGCTT
GGACCTCGCCTTGACCCATCCGATGGTGGTCGTCGAGCTGGCCTGTGGGACACCGCCCACTCCGCGGGAGCACACGCTTG
GCGGTCTGAGCCTGCTGAGGACGTGCAATCAGGCCACGTTGAACGAGGTGCAGGAATTAGTCGAGCGGGAAAGGCTGTAC
GGACTCGGGTGCGGATTGGTCGACATGGCGCTACTTGCATCGACCCTCATCACCCCTGGCGCACGGCTATGGACCCTGGA
CCGGCGCCTGCTGGAGCTGGCGCAGCGATTCGACGTCGCGCATGGCATCCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT236466 WP_004222378.1 NZ_CP092536:c4706557-4706291 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT236466 NZ_LR594663:138503-138709 [Variovorax sp. RA8]
ATGCGTACCACCGTCACCATCGAAGACTCTCTTTTCGAGAAGGCCCTCGAAATGGCCGATCCCGAAATGGACAAGGCGGA
GATCTTTCGAGAAGCGATCCGCACGTTCGTGCGTGTTCAAGCAGGCAAGCGCCTCATTGCGCTCGGAGGGGCGGCCCCCG
ACATCCAGGACGTGCCGCGTCGTCGAGGCGAGCCGGTCTCGTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References