Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4101589..4102208 Replicon chromosome
Accession NZ_CP092536
Organism Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag UPHL_RS19970 Protein ID WP_002892050.1
Coordinates 4101990..4102208 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag UPHL_RS19965 Protein ID WP_002892066.1
Coordinates 4101589..4101963 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UPHL_RS19955 (4096741) 4096741..4097934 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
UPHL_RS19960 (4097957) 4097957..4101103 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
UPHL_RS19965 (4101589) 4101589..4101963 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
UPHL_RS19970 (4101990) 4101990..4102208 + 219 WP_002892050.1 HHA domain-containing protein Toxin
UPHL_RS19975 (4102367) 4102367..4102933 + 567 WP_002892030.1 maltose O-acetyltransferase -
UPHL_RS19980 (4102905) 4102905..4103045 - 141 WP_004147370.1 hypothetical protein -
UPHL_RS19985 (4103066) 4103066..4103536 + 471 WP_002892026.1 YlaC family protein -
UPHL_RS19990 (4103543) 4103543..4105000 - 1458 WP_029883993.1 PLP-dependent aminotransferase family protein -
UPHL_RS19995 (4105101) 4105101..4105799 + 699 WP_002892021.1 GNAT family protein -
UPHL_RS20000 (4105796) 4105796..4105936 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
UPHL_RS20005 (4105936) 4105936..4106199 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236465 WP_002892050.1 NZ_CP092536:4101990-4102208 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T236465 NZ_LR594663:c111936-111511 [Variovorax sp. RA8]
ATGAAAGATAGAGCGTTCGCGGACACCAATGTAGTGCTCTACATGATGTCCAAGGACGCAGCAAAGGCCGAGCGCGCACG
CGCGGTGGTTGCGGGGCGCCCGATCATCAGCGTTCAGGTCCTCAACGAGATTGCCAACGTTGCGCGGCGCAAGCTGACTC
TTCCATGGAAGGAAATCGACGAGTTTCTGCAGTTGGTGCGGGGGCTGTGCCCAGTCGAACCCTTGACCGTTGAATCTCAC
GATCTGGGGCGCGCGCTCAGCGAAAAGTATCAGCTATCGGTGTACGACTCGATGATCTTGTCCTCGGCTCTCCTGTCTGG
CTGCGACACGCTGTATTCCGAGGATATGCAAGACAGTCTGCGCATCGAGAAGACGCTGACCATCGTGAATCCATTTAGCC
TGTACGGGCCCAACTTCGCCCTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT236465 WP_002892066.1 NZ_CP092536:4101589-4101963 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT236465 NZ_LR594663:c112156-111926 [Variovorax sp. RA8]
ATGCAAGTCGCAAAGTGGGGCAACAGTCTGGCCATTCGCCTGCCCCTCGCGCTGGTCAAGAAATTGAACCTGTCAGAAGG
AGACGAGGTGCGCCTGAGCGTGCACAGCCCGCGCGACCTTGGTCTGACCAAGAAGCCCGGGAAGGAGGAGATCGTCGCCG
CCCTGCGTGAGTTCGAAGGCCGGCTGCCAGAAGCGTTCAAGTTCGATCGAGACGACGCCAATGAAAGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References