Detailed information of TA system TA236464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2814303..2815891
• Replicon: chromosome
• Accession: NZ_CP092536
• Organism: Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: UPHL_RS13640
• Protein ID: WP_002904792.1
• Coordinates: 2814303..2815625 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: UPHL_RS13645
• Protein ID: WP_004176198.1
• Coordinates: 2815625..2815891 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UPHL_RS13625 (2809359)	2809359..2811860	+	2502	WP_029884184.1	fimbria/pilus outer membrane usher protein	-
UPHL_RS13630 (2811848)	2811848..2812852	+	1005	WP_029884185.1	fimbrial protein	-
UPHL_RS13635 (2812994)	2812994..2814286	+	1293	WP_016946381.1	glycoside hydrolase family 10 protein	-
UPHL_RS13640 (2814303)	2814303..2815625	-	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
UPHL_RS13645 (2815625)	2815625..2815891	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
UPHL_RS13650 (2816278)	2816278..2816604	-	327	WP_004224757.1	hydroxyisourate hydrolase	-
UPHL_RS13655 (2816601)	2816601..2817101	-	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
UPHL_RS13660 (2817079)	2817079..2818458	-	1380	WP_014599164.1	nucleobase:cation symporter-2 family protein	-
UPHL_RS13665 (2818804)	2818804..2819958	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
UPHL_RS13670 (2819939)	2819939..2820862	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2635537..2858377	222840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T236464 WP_002904792.1 NZ_CP092536:c2815625-2814303 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T236464 NZ_LR594662:3289598-3290470 [Variovorax sp. RA8]
ATGAGCGATGCAGCCGTGATCGACGCCCAGCCCCTGCCGGCATCCGAGCTGGCGCGCCTCGATGCGCGCTTTCCGGCGCG
CAGCGTGAGGGTGCAGGGCGGTGCCGTGGTCTCGGTGCGCGAATGCGGCGCGGGGCCGGCGATCGTGTGCCTGCACGGCA
TCGGCTCGGGTTCGGCCTCGTGGCTGGACACGGCCCTGCTGCTCGAAGGCGAGGCGCGCCTGATCGCCTGGGATGCGCCA
GGCTACGGCGAATCGACGCCGCTGGCAGCGCAGGCGCCCACTGCGCACGACTACGCGCAGCGCCTGCATGGCCTGCTCGA
TGCGCTGGAGATTTCATCGTGCGTGCTGGTCGGCCACTCGCTCGGCGCGCTGATGGCAGCCTCTGCGGCGCATCCGGGCT
CGCCGCTGGCGCCGCGCATCTCAACGCTGGTGCTGATCAGCCCGGCCGCCGGCTACGGCGCCGCGGGCCGTGCAGAGGCG
CGGGCCCGCGTGCGCGCCGAACGCCTGGGCACGCTCGATGAGCTCGGCATCGCCGGCATGGCCGCGAAGCGCTCGGGCCG
GCTGGTCTCTGCGCACGCAAGCGAGACCGCCCGGCAGTGGGTGCGCTGGAACATGGCGCGCTTGCACGAGACAGGTTACC
GCCAGGCCGTGGAGCTGCTGTGCGGCGCCGACCTGCGGGCCGACCTGCCGCCTGCCGTGCCGGTGCGCGTGGCCTGCGGT
GCGCTGGACGTCGTCACTACCCCCGAAGCCTGTGCCGAGGTGGCAAGGCTATGCGCAGTGCCGCTCGAACTGCTGCCCGA
GGCGGGCCACGCGAGCTACGTCGAGCGGCCCGCCGCGGTGGCCGCGCTGCTGCGCGAAGCCATTGCAGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT236464 WP_004176198.1 NZ_CP092536:c2815891-2815625 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT236464 NZ_LR594662:3290498-3291325 [Variovorax sp. RA8]
ATGAGCAACGACCTGATGGAAGAGGGCGCCGATCGTTACAACGTGCCGGCCCTGGAACGCGGCCTTCGCATGCTGTGCGA
GTTCAGCCGCGAGAGCCGCACCCTGTCCGCGCCCGAGATGGCGCGGCGCTTCGACCTGCCGCGCTCCACCGTGTTCCGCC
TGCTCAGCACGCTGGAGAACATGGGCTTTTTGGAGCGCGCAGAGGGTGGCCGCGACTACCGCCTTGGCCTGGCGGTGCTG
CGCCTGGGCTTCGAGTACCTGGCTTCGCTGGAGCTCACGCAGCTGGGCACGCCCTTGCTCAACCGCCTGTGCGAGGAGCT
GCGCACGCCCTGCAACCTGGTGGTGCGCGATGGCCGTTCCATCGTCTACGTGGCCAAGGTCGCGCCGCCCACGCCCTTCG
CGAGCTCGGTCAGCGTCGGCACGCGCCTGCCGGCCCATGCGACCGTGCTGGGCCGCATCCTGCTGGAAGACCTGACGCTG
CCCCAACTGCGCGCGCTCTACCCGGAGGACAAGCTGGAGACCTTCTCGCCGAGCACGCCCAAGACCGTCAACGAGCTCTT
CGACATGGTCCAGGCCGACCGCGAGCGCGGCTACGTGCTGGGCGAGGGCTTCTTCGAATCGAACATCTCCACCATCGCCG
CGCCGGTGCGCGACCACAGCGGCCACATCGCCGCCGCGCTGGGCGCGACCATCACCTCCGGCCACATCGACGAGAACCGC
ATGGACGAGATGGTGCGCCGCGTGCGCGAGACGGCCGACGAGATCTCGGGCCTCCTGAACTACGCGCCCGCCAGCAAGGC
CAAGGTGGTCCCGCTGCGCAGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8