Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 202433..203172 Replicon chromosome
Accession NZ_CP092536
Organism Klebsiella pneumoniae strain 2021CK-00720

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag UPHL_RS00990 Protein ID WP_004901283.1
Coordinates 202687..203172 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag UPHL_RS00985 Protein ID WP_003026799.1
Coordinates 202433..202699 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UPHL_RS00970 (197937) 197937..200006 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
UPHL_RS00975 (200302) 200302..201671 + 1370 WP_087661561.1 IS3 family transposase -
UPHL_RS00980 (201872) 201872..202300 + 429 WP_004901287.1 GFA family protein -
UPHL_RS00985 (202433) 202433..202699 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
UPHL_RS00990 (202687) 202687..203172 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
UPHL_RS00995 (203497) 203497..204543 - 1047 WP_022615678.1 IS481-like element ISKpn28 family transposase -
UPHL_RS01000 (204617) 204617..204769 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
UPHL_RS01005 (205071) 205071..206690 + 1620 WP_029884695.1 ATP-binding cassette domain-containing protein -
UPHL_RS01010 (206789) 206789..207001 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
UPHL_RS01015 (207253) 207253..207543 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 200946..204543 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T236452 WP_004901283.1 NZ_CP092536:202687-203172 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T236452 NZ_LR594051:419236-419544 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT236452 WP_003026799.1 NZ_CP092536:202433-202699 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT236452 NZ_LR594051:418962-419234 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References