Detailed information of TA system TA236449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4147961..4148775
• Replicon: chromosome
• Accession: NZ_CP092534
• Organism: Escherichia coli strain DS566

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: MKD44_RS20175
• Protein ID: WP_001054376.1
• Coordinates: 4147961..4148218 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: S1NWL7
• Locus tag: MKD44_RS20180
• Protein ID: WP_001540600.1
• Coordinates: 4148230..4148775 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD44_RS20150 (4143249)	4143249..4144355	+	1107	WP_001774143.1	N-acetylneuraminate epimerase	-
MKD44_RS20155 (4144420)	4144420..4145400	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
MKD44_RS20160 (4145510)	4145510..4145715	+	206	Protein_3945	HNH endonuclease	-
MKD44_RS20165 (4145983)	4145983..4147223	-	1241	Protein_3946	helicase YjhR	-
MKD44_RS20170 (4147339)	4147339..4147470	+	132	WP_001309182.1	hypothetical protein	-
MKD44_RS20175 (4147961)	4147961..4148218	+	258	WP_001054376.1	YjhX family toxin	Toxin
MKD44_RS20180 (4148230)	4148230..4148775	+	546	WP_001540600.1	N-acetyltransferase	Antitoxin
MKD44_RS20185 (4148831)	4148831..4149577	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
MKD44_RS20190 (4149746)	4149746..4149964	+	219	Protein_3951	hypothetical protein	-
MKD44_RS20195 (4150002)	4150002..4150118	+	117	Protein_3952	VOC family protein	-
MKD44_RS20200 (4150363)	4150363..4151484	+	1122	WP_000010829.1	M42 family metallopeptidase	-
MKD44_RS20205 (4151481)	4151481..4151759	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
MKD44_RS20210 (4151771)	4151771..4153084	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4137747..4156999	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T236449 WP_001054376.1 NZ_CP092534:4147961-4148218 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T236449 NZ_LR594048:c2144231-2143962 [Streptococcus dysgalactiae subsp. equisimilis]
ATGACTTATAAGCTAGTGGTGAGTGATGAAGTCAAGAAGCAACTCAAAAAGATGGATAAGCATGTAGGGCTTATGTTAGC
CAAAGACATGAAGAAACGACTTGACGGGTTAAATAATCCTAGACAATTTGGAAAAGCCCTCACAGGTCAATATAAAGGGC
TTTGGCGCTATCGTGTTGGTAATTATCGGGTGATTTGTGACATTGTGGATAATGAAATGATTATCCTTGCTTTGGAAGTT
GGACACAGGAAAGAGATATACAAGCGCTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19926.87 Da
• Isoelectric Point: 6.3277

>AT236449 WP_001540600.1 NZ_CP092534:4148230-4148775 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT236449 NZ_LR594048:c2144448-2144221 [Streptococcus dysgalactiae subsp. equisimilis]
ATGGCAATGGTATCATTAAGACTGAACAGTGCAGAGGAAGAGCTTTTCAGAAGCTACGCTACACATACAGGCAAAACATT
ATCTGAATTATTCAAGACAGCACTAGCGCAACAAATTGAAGATCAATTGGACTATGAAACAGGTATTCAAGCTTTAAAAC
GCTTTGAAGAAAATCCTATAACTTATTCTATCGATGATTTAATTGAGGAGTTGGAAAATGACTTATAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: S1NWL7