Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3519913..3520531 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MKD44_RS17185 Protein ID WP_001291435.1
Coordinates 3520313..3520531 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MKD44_RS17180 Protein ID WP_000344800.1
Coordinates 3519913..3520287 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS17170 (3515002) 3515002..3516195 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKD44_RS17175 (3516218) 3516218..3519367 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
MKD44_RS17180 (3519913) 3519913..3520287 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MKD44_RS17185 (3520313) 3520313..3520531 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MKD44_RS17190 (3520704) 3520704..3521255 + 552 WP_000102539.1 maltose O-acetyltransferase -
MKD44_RS17195 (3521371) 3521371..3521841 + 471 WP_000136192.1 YlaC family protein -
MKD44_RS17200 (3522005) 3522005..3523555 + 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MKD44_RS17205 (3523597) 3523597..3523950 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MKD44_RS17215 (3524329) 3524329..3524640 + 312 WP_000409908.1 MGMT family protein -
MKD44_RS17220 (3524671) 3524671..3525243 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236440 WP_001291435.1 NZ_CP092534:3520313-3520531 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T236440 NZ_LR594045:c242067-241732 [Streptococcus dysgalactiae subsp. equisimilis]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGTTAAAGGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCGACATCAGGTCAGCACAAAATGGAGCAAATCATTAGTGACATAGAAAAACTCGAGGTTTTCCCTGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGTAAGTACCACAGCACTAGAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGAGGAAGAGAATAGGGTCGTTATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT236440 WP_000344800.1 NZ_CP092534:3519913-3520287 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT236440 NZ_LR594045:c242344-242057 [Streptococcus dysgalactiae subsp. equisimilis]
ATGGTTACAGCAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAGAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTTCAAAATGTCGTTGTCACAAATGAGGTTGACTTATTGA
CGGAAGAGGAGCTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAAGATGTTCGTCAA
GGGAAATTTTATACCTCTGAAGAAGTGAGGTCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References