Detailed information of TA system TA023643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3756241..3756770
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JTY_RS17620
• Protein ID: WP_003417760.1
• Coordinates: 3756513..3756770 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JTY_RS17615
• Protein ID: WP_003417757.1
• Coordinates: 3756241..3756516 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS17580	3751272..3752570	-	1299	WP_011799343.1	PPE family protein	-
JTY_RS22600	3753039..3754476	-	1438	Protein_3438	FAD-binding oxidoreductase	-
JTY_RS17600	3754579..3754968	+	390	WP_010950876.1	DUF732 domain-containing protein	-
JTY_RS17605	3754982..3755275	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JTY_RS17610	3755272..3756117	-	846	WP_010950877.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JTY_RS17615	3756241..3756516	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JTY_RS17620	3756513..3756770	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JTY_RS17625	3756812..3758002	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JTY_RS17630	3758119..3758487	+	369	WP_003417765.1	FHA domain-containing protein	-
JTY_RS17635	3758484..3759035	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JTY_RS17640	3759042..3759623	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JTY_RS17645	3759604..3759972	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JTY_RS17650	3759950..3760342	-	393	WP_011799344.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T23643 WP_003417760.1 NC_012207:3756513-3756770 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T23643 NC_012207:3756513-3756770 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT23643 WP_003417757.1 NC_012207:3756241-3756516 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT23643 NC_012207:3756241-3756516 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94