Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1089456..1090039 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag MKD44_RS05155 Protein ID WP_000254749.1
Coordinates 1089704..1090039 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MKD44_RS05150 Protein ID WP_000581937.1
Coordinates 1089456..1089704 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS05140 (1085795) 1085795..1087096 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MKD44_RS05145 (1087144) 1087144..1089378 + 2235 WP_000226797.1 GTP diphosphokinase -
MKD44_RS05150 (1089456) 1089456..1089704 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MKD44_RS05155 (1089704) 1089704..1090039 + 336 WP_000254749.1 endoribonuclease MazF Toxin
MKD44_RS05160 (1090111) 1090111..1090902 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MKD44_RS05165 (1091130) 1091130..1092767 + 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
MKD44_RS05170 (1092855) 1092855..1094153 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T236428 WP_000254749.1 NZ_CP092534:1089704-1090039 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T236428 NZ_LR594038:2155718-2155821 [Klebsiella grimontii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT236428 WP_000581937.1 NZ_CP092534:1089456-1089704 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT236428 NZ_LR594038:c2155828-2155660 [Klebsiella grimontii]
AATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGTGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References