Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933458..934112 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MKD44_RS04530 Protein ID WP_000244781.1
Coordinates 933705..934112 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MKD44_RS04525 Protein ID WP_000354046.1
Coordinates 933458..933724 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS04505 (929546) 929546..930979 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
MKD44_RS04510 (931024) 931024..931335 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
MKD44_RS04515 (931499) 931499..932158 + 660 WP_000250274.1 hemolysin III family protein -
MKD44_RS04520 (932235) 932235..933215 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MKD44_RS04525 (933458) 933458..933724 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MKD44_RS04530 (933705) 933705..934112 + 408 WP_000244781.1 protein YgfX Toxin
MKD44_RS04535 (934152) 934152..934673 - 522 WP_001055874.1 flavodoxin FldB -
MKD44_RS04540 (934785) 934785..935681 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MKD44_RS04545 (935706) 935706..936416 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MKD44_RS04550 (936422) 936422..938155 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T236427 WP_000244781.1 NZ_CP092534:933705-934112 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T236427 NZ_LR594038:1951203-1951556 [Klebsiella grimontii]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGCTCAACGATACCGATAGGGCCAGGGTGCTTGC
AGCGTTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTGAGAGGCTCCCGCTACAGCG
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGTAATAAGGTTGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGAGCGGGAATTCAC
GAACTGGCTGAAGACATTCAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236427 WP_000354046.1 NZ_CP092534:933458-933724 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236427 NZ_LR594038:1951561-1951863 [Klebsiella grimontii]
ATGGGCAGAACGTTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAGATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCGGATGGTTCTCACTACGGATTTGAACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References