Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4417813..4418310 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MKD47_RS21550 Protein ID WP_001173343.1
Coordinates 4417813..4417986 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MKD47_RS21555 Protein ID WP_001312331.1
Coordinates 4418014..4418310 (-) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS21540 (4414483) 4414483..4415940 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
MKD47_RS21545 (4416177) 4416177..4417694 + 1518 WP_000003806.1 lysine--tRNA ligase -
MKD47_RS21550 (4417813) 4417813..4417986 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MKD47_RS21555 (4418014) 4418014..4418310 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MKD47_RS21560 (4418539) 4418539..4418811 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MKD47_RS21565 (4418823) 4418823..4419053 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
MKD47_RS21570 (4419234) 4419234..4420865 + 1632 WP_001216482.1 sensor histidine kinase -
MKD47_RS21575 (4420862) 4420862..4421581 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4361914..4418310 56396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T236423 WP_001173343.1 NZ_CP092533:c4417986-4417813 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T236423 NZ_LR594037:c1280096-1279731 [Streptococcus anginosus]
GTGCATAATATCTATTTTTACAAAGATAAAAACGGGAATGAGCCGGTTTTAGATTATATGCGTGAACTTGCCAGCAAAAA
AGGCAAGGATAGTCGTATCAAACTAAACAAGATAAATGACTATATCGAACTACTAAGCCAACGTGGTACACGCGCAGGAG
AACCGTATATTAAGCATTTAGATGCTGAAATTTGGGAGCTTAGACCATTGAGGGATAGGATATTATTTGTTGCTTGGATT
GATGGCAACTTTGTATTGTTGCATCATTTTATGAAAAAGACGCAGAAAACACCAAAACGAGAAATTGAACAAGCAAAGCG
TGAATTACGCGACTTAAAAGAAAGAGGGCTATACAATGAAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT236423 WP_001312331.1 NZ_CP092533:c4418310-4418014 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT236423 NZ_LR594037:c1279741-1279445 [Streptococcus anginosus]
ATGAAAAATAGTGCAATTGGTAGTAACTGGAAAGATGTGAGAGCTGAACTTTTTTCCAAAGAGGAAATTTTGGAGAGTGA
TATGCGCGTGGCAATCATGAGTGAGCTGATTGATGCTAGAAATGAAAAAGGGATCAGTCAGAAAAAACTTGAGGAACTAA
GCGGAGTAAGTCAGCCAGTTATTGCTAGAATGGAAACAGGTAAGACAAGTCCACAACTTGATACTGTTTTAAAAGTCTTG
GCAAGTCTTGGGAAAACTTTGGCAGTTGTACCCTTAGAGGGCGAACAGGTAAGCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References