Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3994076..3994626 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MKD47_RS19425 Protein ID WP_001160966.1
Coordinates 3994076..3994390 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag MKD47_RS19430 Protein ID WP_000125568.1
Coordinates 3994393..3994626 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS19400 (3989751) 3989751..3991037 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
MKD47_RS19405 (3991037) 3991037..3992026 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MKD47_RS19410 (3992023) 3992023..3992844 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MKD47_RS19415 (3992847) 3992847..3993224 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MKD47_RS19420 (3993231) 3993231..3994079 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MKD47_RS19425 (3994076) 3994076..3994390 - 315 WP_001160966.1 CcdB family protein Toxin
MKD47_RS19430 (3994393) 3994393..3994626 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MKD47_RS19435 (3994712) 3994712..3995191 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
MKD47_RS19440 (3995383) 3995383..3997245 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
MKD47_RS19445 (3997238) 3997238..3997768 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MKD47_RS19450 (3997876) 3997876..3999207 - 1332 WP_001183207.1 MFS transporter -
MKD47_RS19455 (3999266) 3999266..3999553 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T236414 WP_001160966.1 NZ_CP092533:c3994390-3994076 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T236414 NZ_LR590625:981733-982068 [Streptococcus canis]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGTTAAAGGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCGACATCAGGTCAGCACAAAATGGAGCAAATCATTAGTGACATAGAAAAACTCAAGGTTTTCCCTGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGTAAGTACCACAGCACTAGAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGAGGAAGAGAATAGGGTCGTTATTGATTACTTGCTTCCTGCTCGAAGCGACTATAT
CAAATTATTCAAATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT236414 WP_000125568.1 NZ_CP092533:c3994626-3994393 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT236414 NZ_LR590625:981456-981743 [Streptococcus canis]
ATGGTTACAGTAGAAAAAAATAGAGCTGTTACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAGAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTTCAAAATGTCGTTGTCACAAATGAGGTTGACTTATTGA
CGGAAGAGGAGCTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAAGATGTTCGTCAA
GGGAAATTTTATACCTCTGAAGAAGTGAGGTCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References