236400

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1089434..1090017 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag MKD47_RS05155 Protein ID WP_000254749.1
Coordinates 1089682..1090017 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MKD47_RS05150 Protein ID WP_000581937.1
Coordinates 1089434..1089682 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS05140 (1085773) 1085773..1087074 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MKD47_RS05145 (1087122) 1087122..1089356 + 2235 WP_000226797.1 GTP diphosphokinase -
MKD47_RS05150 (1089434) 1089434..1089682 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MKD47_RS05155 (1089682) 1089682..1090017 + 336 WP_000254749.1 endoribonuclease MazF Toxin
MKD47_RS05160 (1090089) 1090089..1090880 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MKD47_RS05165 (1091108) 1091108..1092745 + 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
MKD47_RS05170 (1092833) 1092833..1094131 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T236400 WP_000254749.1 NZ_CP092533:1089682-1090017 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T236400 NZ_LR590482:c1945797-1945225 [Pseudomonas synxantha]
ATGGACCTGGAAAAAATCAAAGGCCGTCTCGATTTCCTGCGCGAAGCGGAAAAACTCAAGGATGTGCTCAGAAGCGCCCA
CACGGCTTCGGGTCGCACTGAAAGCACGGCCGAACACAGTTGGCGCCTGTGTCTGATGGCGATGGTATTCGCCGATCAAC
TGCCCGGCCTGGACCTGCTGAAAATCCTCAAGATGTGCGTTATCCATGATTTGGGCGAAGCCATCCACGGCGATATCCCG
GCGGTTAGCCAGGCGGCCTTTCCCAACAAGGGCGAGCAGGAGCGGCTCGACCTGCTGCTGTTGGCCCAGTCCCTGGACAG
TCCGCTGAAAACCGAGATCCTGACGTTGTGGGATGACTACGAGAACGCGCTTTCGCCGGAAGCAAAAGCCGTCAAAGCCA
TGGACAAACTGGAGACCCTGCTGCAGCACACCCAGGGGCTGAACCCTCCCGATTTCGACTACGCCTTCAACCTGGCCTAC
GGCAAAAAACACACGGATGCCGACCCCTTGTTCGAAGCATTGCGGGCATTGATCGATCGAGCGACAGAGCAAAGGCTTCA
CGCCGCCGAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT236400 WP_000581937.1 NZ_CP092533:1089434-1089682 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT236400 NZ_LR590482:c1946442-1945885 [Pseudomonas synxantha]
ATGCCTCACTCCTTGCTCAAGCCTGCGCTGCCCGGCATTGCGCTGCGGCGATGGCGTTTGCTGCACCGCGTCAAGCAAAG
CCATGCGGCGCAGCTATTCAACGTCACTCAATCGACTATTTCGCGCTGGGAAAGTGGCGTACAAGCGATGGATCCGGCTG
CGCACCAACAGCTTGAGCAGTTGTTGGCAGCCCGGCTTGATGCAGCCGCCGACCAGGCATTGGCCCGGCTGGTCAGTGAG
AGTTCGCAAGCGATGCACCTGGTGTGCGACCTGACGCACCGCCTGCTCGCATGCTCGCCCTCCCGCGCACTCCAATTCAC
CAGCCCGCTCGCCGATCTTATGGGCCAGTCGCTGTGGCGCTTCGCAACCCAGGAAATCCAGCTGATCGAAGCCAGCCTGG
AACACACCGGCTGGCGCGAAACCCAGGCCCCCCCAGCCCTTGAGTTCACCACTGGAAGCAACGATTCCAGCCTCGTGCCG
ATACGCCCAAGCCTGTGTCGCTGGACACGCATGACCCTGAGCGATGGCAGCGCCGCGCGCCTGGTCGAGACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References