Detailed information of TA system TA023640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3129897..3130584
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FBY8
• Locus tag: JTY_RS14865
• Protein ID: WP_011799298.1
• Coordinates: 3130141..3130584 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: JTY_RS14860
• Protein ID: WP_003414620.1
• Coordinates: 3129897..3130154 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS14840	3125217..3126071	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
JTY_RS14845	3126127..3127290	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
JTY_RS14850	3127307..3128521	-	1215	WP_011799297.1	zinc metalloprotease Rip	-
JTY_RS14855	3128529..3129770	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
JTY_RS14860	3129897..3130154	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
JTY_RS14865	3130141..3130584	+	444	WP_011799298.1	type II toxin-antitoxin system VapC family toxin	Toxin
JTY_RS14870	3130664..3131326	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
JTY_RS14875	3131422..3131613	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JTY_RS14880	3131945..3133693	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
JTY_RS14885	3133789..3134370	+	582	WP_003414644.1	fasciclin domain-containing protein	-
JTY_RS14890	3134470..3134736	+	267	WP_031657293.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16625.74 Da
• Isoelectric Point: 6.4890

>T23640 WP_011799298.1 NC_012207:3130141-3130584 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T23640 NC_012207:3130141-3130584 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACACCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT23640 WP_003414620.1 NC_012207:3129897-3130154 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT23640 NC_012207:3129897-3130154 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBY8

Antitoxin

• AlphaFold DB: G0TFH0