Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933436..934090 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MKD47_RS04530 Protein ID WP_000244781.1
Coordinates 933683..934090 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MKD47_RS04525 Protein ID WP_000354046.1
Coordinates 933436..933702 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS04505 (929524) 929524..930957 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
MKD47_RS04510 (931002) 931002..931313 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
MKD47_RS04515 (931477) 931477..932136 + 660 WP_000250274.1 hemolysin III family protein -
MKD47_RS04520 (932213) 932213..933193 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MKD47_RS04525 (933436) 933436..933702 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MKD47_RS04530 (933683) 933683..934090 + 408 WP_000244781.1 protein YgfX Toxin
MKD47_RS04535 (934130) 934130..934651 - 522 WP_001055874.1 flavodoxin FldB -
MKD47_RS04540 (934763) 934763..935659 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MKD47_RS04545 (935684) 935684..936394 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MKD47_RS04550 (936400) 936400..938133 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T236399 WP_000244781.1 NZ_CP092533:933683-934090 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T236399 NZ_LR590482:1191216-1191629 [Pseudomonas synxantha]
GTGACGACATACATGCTGGATACCTGCATTTGCTCATTCATCATGCGTGAACGGCCAGAGTGTGTGCTTGAGCGACTGGC
ATTGGAGGTCGCGAGGAGCAATCGAATCGTGATTTCGGCCATAACCTATGCCGAAATGCGATATGGACAGATTGGGAAAA
AGGCTTCGCCCAAACACAAGACGTTAGTCGATGAGTTTGTGAAGCGGCTCGATGAGGTATTGCCTTGGGGCTTGGCAGCG
GTGGACGCGACGGTTGAGGTGAAGCGAAGCTTGACTGGGCAAGGGTCGATCATCGGTCAAAATGACACGGCGATAGCCGG
GCATGCAATTGCTGCGGGATGTACGTTGGTAACCAACAACGTCCGGGAATTCAGTCGTGTAGCGGGGTTGTCTTACGAGG
ACTGGGTTCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236399 WP_000354046.1 NZ_CP092533:933436-933702 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236399 NZ_LR590482:1190989-1191219 [Pseudomonas synxantha]
ATGCGCACAGTCTCGATTTTTAAAAACGGAAAGAACCAGGCTATTCGATTCCCAAACGACATGGCCTATGAGGGAGTCGG
CGAACTCGAAATCACCCGCAACGGAGATACGATTACGTTGCGACCTGTACGACCTAGCTGGGGGTCGTTGCGTGAACTGC
CCAAGGCGGACGAAGATTTTCTACAGGAGCGTCAGGACGTTATTGGCGATGAAGGCAGGTTCAATCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References