Detailed information of TA system TA236394

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4406140..4406972
• Replicon: chromosome
• Accession: NZ_CP092532
• Organism: Escherichia coli strain DS566-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: MKD45_RS21510
• Protein ID: WP_000854753.1
• Coordinates: 4406598..4406972 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: MKD45_RS21505
• Protein ID: WP_001540478.1
• Coordinates: 4406140..4406508 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD45_RS21470 (4401973)	4401973..4402653	+	681	WP_001282919.1	WYL domain-containing protein	-
MKD45_RS21475 (4402801)	4402801..4403478	+	678	WP_001097301.1	hypothetical protein	-
MKD45_RS21480 (4403484)	4403484..4403717	+	234	WP_001278283.1	DUF905 family protein	-
MKD45_RS21485 (4403807)	4403807..4404625	+	819	WP_001773857.1	DUF932 domain-containing protein	-
MKD45_RS21490 (4404716)	4404716..4405201	+	486	WP_000214398.1	antirestriction protein	-
MKD45_RS21495 (4405217)	4405217..4405693	+	477	WP_001186786.1	RadC family protein	-
MKD45_RS21500 (4405756)	4405756..4405977	+	222	WP_000692311.1	DUF987 domain-containing protein	-
MKD45_RS21505 (4406140)	4406140..4406508	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MKD45_RS21510 (4406598)	4406598..4406972	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
MKD45_RS21515 (4406969)	4406969..4407457	+	489	WP_000777545.1	DUF5983 family protein	-
MKD45_RS21520 (4407469)	4407469..4407666	+	198	WP_000839281.1	DUF957 domain-containing protein	-
MKD45_RS21525 (4407763)	4407763..4408332	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
MKD45_RS21530 (4409081)	4409081..4410619	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4393849..4418432	24583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T236394 WP_000854753.1 NZ_CP092532:4406598-4406972 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T236394 NZ_LR590480:c1364551-1364369 [Bordetella parapertussis]
ATGGACGGCAAAGAACTGATCAAGCAGCTTGAGCGGTCCGGGTGGACACTGCGGAGCGTTAAGGGCTCCCACCACGTTTT
CGCCCACCCAGACCGCCCCGGACATATCAGCGTCCCTCACCCTAAAAAGGATCTCGGCATCGGACTACTAAACAGCATCC
TCAAAGCAGCTGGTTTGAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT236394 WP_001540478.1 NZ_CP092532:4406140-4406508 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT236394 NZ_LR590480:c1364307-1363915 [Bordetella parapertussis]
GTGAAATATCCCATTGCCATTGAACCCGGCAGCGACACGCGCGCCTGGGGAGTCGTTGTTCCTGATCTGCCCGGCTGCTT
TTCCGCAGCGGACGAAGGGATTGATCAAGCAATCGAGAACGCCAAGGAAGCCATTGAGTTGTGGATTGAAACGGCCATCG
ACTCCGGCGCCCCCATTCCTGCGGCAACCAACATAGCCAATCATCAGGCCAATCCTGAGTTCGCCAACTGGATTTGGGCG
ATTGCTGATGTCGATCCTGCAGTCTTGGATGAAACTGTCGAGCGCGTGAACATCACAATCCCGCGCCGAATTCTCAAGCG
CCTGGATGATCGCGCCCGCGCCGCCGGCGAAAGCCGGAGCAGCTACATCGCACATCTCGCCATGACTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68