Detailed information of TA system TA236393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4147907..4148721
• Replicon: chromosome
• Accession: NZ_CP092532
• Organism: Escherichia coli strain DS566-2

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: MKD45_RS20175
• Protein ID: WP_001054376.1
• Coordinates: 4147907..4148164 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: S1NWL7
• Locus tag: MKD45_RS20180
• Protein ID: WP_001540600.1
• Coordinates: 4148176..4148721 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD45_RS20150 (4143195)	4143195..4144301	+	1107	WP_001774143.1	N-acetylneuraminate epimerase	-
MKD45_RS20155 (4144366)	4144366..4145346	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
MKD45_RS20160 (4145456)	4145456..4145661	+	206	Protein_3945	HNH endonuclease	-
MKD45_RS20165 (4145929)	4145929..4147169	-	1241	Protein_3946	helicase YjhR	-
MKD45_RS20170 (4147285)	4147285..4147416	+	132	WP_001309182.1	hypothetical protein	-
MKD45_RS20175 (4147907)	4147907..4148164	+	258	WP_001054376.1	YjhX family toxin	Toxin
MKD45_RS20180 (4148176)	4148176..4148721	+	546	WP_001540600.1	N-acetyltransferase	Antitoxin
MKD45_RS20185 (4148777)	4148777..4149523	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
MKD45_RS20190 (4149692)	4149692..4149910	+	219	Protein_3951	hypothetical protein	-
MKD45_RS20195 (4149948)	4149948..4150064	+	117	Protein_3952	VOC family protein	-
MKD45_RS20200 (4150309)	4150309..4151430	+	1122	WP_000010829.1	M42 family metallopeptidase	-
MKD45_RS20205 (4151427)	4151427..4151705	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
MKD45_RS20210 (4151717)	4151717..4153030	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4137693..4156945	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T236393 WP_001054376.1 NZ_CP092532:4147907-4148164 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T236393 NZ_LR590477:c807870-807451 [Neisseria lactamica]
ATGATTTTACTGGATACAAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATGACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCCCGACGGCA
AGAAAAAGAATGTGCTGCACGAGCGTTTGGAACAATCCATTTTGCCTTTATTTGCGAGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGCGATTGCCGCCGCAGACGGCTATAT
TGCAGCAACGGCCAAACAGCACCGTTTGACAGTTGCTACGCGTGATACCGGCCCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGTACGATTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19926.87 Da
• Isoelectric Point: 6.3277

>AT236393 WP_001540600.1 NZ_CP092532:4148176-4148721 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT236393 NZ_LR590477:c808106-807870 [Neisseria lactamica]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGAGCACGCACAACGCACTCAAATTCCGCGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATCTTGGATAACATCGCCAAAGCACAGCAAACGGTACGTTTGGGGTCAATGTTGGCAT
CAATCGGGCAGGAAGTGGATGGTGTGGAGTTGGATAACGTGCGTGATTTCGATACCAAGAATGAGGTATCCTTTTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: S1NWL7